B8HGW2 (RSMH_ARTCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	Achl_1563

Organism

Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]

Taxonomic identifier

452863 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000386719
Regions
	Region	48 – 50	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	67	1	S-adenosyl-L-methionine By similarity
	Binding site	101	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	115	1	S-adenosyl-L-methionine By similarity
	Binding site	122	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8HGW2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: D9A27D9FFACCD30D	FASTA	330	36,035
	10 20 30 40 50 60 MNEQPKPTSE RHVPVLRDRC INLLAPGFDA ARQQGKAPIV IDATLGMGGH SEAMLQRFPD 70 80 90 100 110 120 LHLIGIDRDE EALALAGERL APFSARTDLV HAVYDEIPEV LADLGVTEIS GILMDLGVSS 130 140 150 160 170 180 LQLDERERGF AYSFDAPLDM RMDTSRGQTA ADVVNTYNEE ELVRIIRKWG EEKFAGRIAN 190 200 210 220 230 240 RIVAARGEKP FTTTGELVEQ IRSVVPAGAA KSGGHPAKRT FQALRIEVNE ELDVLERAVP 250 260 270 280 290 300 AAVDALAMGG RIVVMSYHSL EDKIVKSVLQ GRSKSSAPLG FPVELEEHKP ELKILTKGTE 310 320 330 VPTAVEIAEN PRAASARLRA AERIRARRAA

References

[1]

"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001341 Genomic DNA. Translation: ACL39551.1.
RefSeq	YP_002487640.1. NC_011886.1.
3D structure databases
ProteinModelPortal	B8HGW2.
ModBase	Search...
Protein-protein interaction databases
STRING	B8HGW2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7293016.
GenomeReviews	Gene locus Achl_1563 in contig CP001341_GR.
KEGG	ach:Achl_1563.
PATRIC	20989687. VBIArtChl38304_2234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_ARTCA

Accession

Primary (citable) accession number: B8HGW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program