B8HGQ9 (B8HGQ9_ARTCA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 2 HAMAP MF_00012
Short name=DAD 2 HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD2 HAMAP MF_00012
Ordered Locus Names:	Achl_3368

Organism

Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]

Taxonomic identifier

452863 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL ACL41325.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	196	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	B8HGQ9 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 964E0D7FABE3E98C	FASTA	619	64,897
	10 20 30 40 50 60 MPALRSKTVT HGRNMAGARA LLRASGVANS DIGKPIIAVA NSFTEFVPGH THLAPVGRIV 70 80 90 100 110 120 SDAILAAGAV PREFNTIAVD DGIAMGHSGM LYSLPSRDLI ADSVEYMVNA HCADALVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPV VFVSGGPMEA GRVTLTDGSV RSLDLVNAIA DAVDESISDE 190 200 210 220 230 240 DINLIEENAC PTCGSCSGMF TANSMNCLAE AIGLALPGNG SVLATHTARK ALYEKAGATV 250 260 270 280 290 300 VELVKRYYDG DDDSVLPRNI ATAEAFDNAM ALDISMGGST NTILHLLAAA QEAGVDYGLA 310 320 330 340 350 360 EMDAKSRQVP CLAKVAPNVA GNKTYYMEDV HRAGGIPALL GELNRGGLLH KNVHSVHSTD 370 380 390 400 410 420 LDGWLDDWDI RGGKATEEAQ ALWHAAPGGV RSSTAFSQSN EWTSLDTDAA EGCIRSVEHA 430 440 450 460 470 480 FSKDGGLAVL RGNVAEDGAV VKTAGVDESI WTFSGPAVVC ESQDEAVEKI LNKGIKEGDV 490 500 510 520 530 540 VVIRYEGPKG GPGMQEMLYP TSFLKGRGLG KKCALITDGR FSGGTSGLSI GHISPEAASG 550 560 570 580 590 600 GTIALVEDGD IISINISQRS LQLEVSDELL AERREKLLVN GGYKPKDRDR HVSAALRAYA 610 AMATSADKGA VRDVSLLER

References

[1]

"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001341 Genomic DNA. Translation: ACL41325.1.
RefSeq	YP_002489414.1. NC_011886.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8HGQ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7294849.
GenomeReviews	Gene locus Achl_3368 in contig CP001341_GR.
KEGG	ach:Achl_3368.
PATRIC	20993369. VBIArtChl38304_4047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8HGQ9_ARTCA

Accession

Primary (citable) accession number: B8HGQ9

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)