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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Arthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / JCM 12360 / NCIMB 13794 / A6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi519 – 5191MagnesiumUniRule annotation
Metal bindingi525 – 5251MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciACHL452863:GH1A-1465-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Achl_1439
OrganismiArthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / JCM 12360 / NCIMB 13794 / A6)
Taxonomic identifieri452863 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
ProteomesiUP000002505 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752Polyribonucleotide nucleotidyltransferasePRO_1000185718Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi452863.Achl_1439.

Structurei

3D structure databases

ProteinModelPortaliB8HG67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini585 – 64460KHUniRule annotationAdd
BLAST
Domaini656 – 72873S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8HG67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGPEIQFSE AVIDNGRFGK RVIRFETGRL AKQAAGAAMV YIDEDTALLS
60 70 80 90 100
ATTAGKHPRE GFDFFPLTVD VEERMYAAGR IPGSFFRREG RPSTEAILAC
110 120 130 140 150
RLMDRPLRPA FVKGLRNEVQ IVVTVLAINP DELYDVVAIN ASSMSTQLSG
160 170 180 190 200
LPFSGPIGGV RVALVADENG SQWVAFPKHS QLENSVFNMV VAGRVAGDDV
210 220 230 240 250
AIMMVEAEAT DNSWNLIKEQ GATAPTEEVV SEGLEAAKPF IKALCDAQAD
260 270 280 290 300
LAARAAKPTV EFPVFLDYQD DVYAAVEAAA ADKLAAVFQI ADKQERDTAS
310 320 330 340 350
DALKDEVTSS LAGQFEGREK ELSAAFRSVT KHVVRQRILK DQIRIDGRGL
360 370 380 390 400
TDIRQLTAEV EVLPRVHGSA IFERGETQIM GVTTLNMLKM EQQIDSLSPV
410 420 430 440 450
TRKRYMHNYN FPPYSTGETG RVGSPKRREI GHGALAERAL VPVLPSREEF
460 470 480 490 500
PYAIRQVSEA LGSNGSTSMG SVCASTLSML NAGVPLKAAV AGIAMGLVSD
510 520 530 540 550
QVDGQTRYAA LTDILGAEDA FGDMDFKVAG TSEFVTAIQL DTKLDGIPAS
560 570 580 590 600
VLAAALKQAR EARLHILEVI NSAIDTPDEL SEFAPRVIAV KIPVDKIGEV
610 620 630 640 650
IGPKGKMINQ IQEDTGADIS IEDDGTVYIG ATNGPSADAA RSAINAIANP
660 670 680 690 700
QVPEIGERYL GTVVKTTTFG AFVSLTPGKD GLLHISELRK LANGKRVDNV
710 720 730 740 750
DDVVSVGQKV QVEITKIDDR GKLSLSPVVA EEEGAEGAER AHATEPAEGA

EI
Length:752
Mass (Da):80,574
Last modified:March 3, 2009 - v1
Checksum:i588C18EAA6EE4AD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001341 Genomic DNA. Translation: ACL39429.1.
RefSeqiYP_002487518.1. NC_011886.1.

Genome annotation databases

EnsemblBacteriaiACL39429; ACL39429; Achl_1439.
KEGGiach:Achl_1439.
PATRICi20989419. VBIArtChl38304_2104.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001341 Genomic DNA. Translation: ACL39429.1.
RefSeqiYP_002487518.1. NC_011886.1.

3D structure databases

ProteinModelPortaliB8HG67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi452863.Achl_1439.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL39429; ACL39429; Achl_1439.
KEGGiach:Achl_1439.
PATRICi20989419. VBIArtChl38304_2104.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciACHL452863:GH1A-1465-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Arthrobacter chlorophenolicus A6."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700700 / DSM 12829 / JCM 12360 / NCIMB 13794 / A6.

Entry informationi

Entry nameiPNP_ARTCA
AccessioniPrimary (citable) accession number: B8HG67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 29, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.