B8HG47 (B8HG47_ARTCA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase HAMAP MF_01569
EC=6.1.1.15 HAMAP MF_01569

Alternative name(s):
Prolyl-tRNA synthetase HAMAP MF_01569

Gene names

Name:	proS HAMAP MF_01569
Ordered Locus Names:	Achl_1419

Organism

Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]

Taxonomic identifier

452863 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily. HAMAP MF_01569

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_01569
Cellular component	Cytoplasm HAMAP MF_01569
Ligand	ATP-binding HAMAP MF_01569 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_01569 EMBL ACL39409.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B8HG47 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: A9282942D1FDD298	FASTA	599	64,225
	10 20 30 40 50 60 MSQLFLRTLR EDPVDAEVAS HKLLVRAGYI RRAAPGIYTW LPLGLSVLRK VEQVIREEMA 70 80 90 100 110 120 AIGAQEVHFP ALLPREPYEA TNRWTEYGEG LFRLQDRKGA DYLLAPTHEE MFTLLVKDLY 130 140 150 160 170 180 SSYKDLPLSL YQIQNKYRDE ARPRAGLLRG REFIMKDSYS FDVDDAGLDA SYAAHRSAYL 190 200 210 220 230 240 KIFERLGLDV VPVAATAGAM GGSRSEEFLF PTEIGEDTFV RSAAGYAANV EAVTTVVPAD 250 260 270 280 290 300 IDFTGAPAAE VLDTPDTPTI ETLVNASNQI APRAEADGGP WTAADTLKNV VLAVTLPTGE 310 320 330 340 350 360 RQLVVIGLPG DRGVDLKRVE ANIGSFLPMA GEIGLEQAGE EDLKKQPLIV KGYLGPGLSL 370 380 390 400 410 420 DAPLLGSEGT TKLLYLVDPR VVSGTSWITG ANEAGKHVFG LVAGRDFTWD GVIECTEVRA 430 440 450 460 470 480 GDPAPDGSGP LETARGIEMG HIFQLGRKYA AALDLKVLDQ NGKQVTVTMG SYGVGVTRAV 490 500 510 520 530 540 AALAEANHDA KGLTWPRAVA PADVHVVAVG KGEDIFAAAE QLSVGLEAAG LDVIYDDRFK 550 560 570 580 590 VSPGVKFGDA ELLGVPTILA VGRGLVDGVV EIKDRRTGEA ENVAVDKAVD YVVNAVRNS

References

[1]

"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001341 Genomic DNA. Translation: ACL39409.1.
RefSeq	YP_002487498.1. NC_011886.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8HG47.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7292868.
GenomeReviews	Gene locus Achl_1419 in contig CP001341_GR.
KEGG	ach:Achl_1419.
PATRIC	20989373. VBIArtChl38304_2081.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8HG47_ARTCA

Accession

Primary (citable) accession number: B8HG47

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)