B8HFR2 (DXR_ARTCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	Achl_1411

Organism

Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]

Taxonomic identifier

452863 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_1000189854
Regions
	Nucleotide binding	9 – 38	30	NADP By similarity
Sites
	Metal binding	156	1	Divalent metal cation By similarity
	Metal binding	158	1	Divalent metal cation By similarity
	Metal binding	227	1	Divalent metal cation By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8HFR2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 984211F1432ECB25	FASTA	394	40,465
	10 20 30 40 50 60 MQPRRIAILG STGSIGTQAI DVVDGAPHLF EVVALSAGGG NLALLARQAV HTGAAAVGIA 70 80 90 100 110 120 AGDPRELAVL IDEAAAAAGR SGYRPEIIAG PDASTRIAGL DADVVLNGIT GSIGLAPTLA 130 140 150 160 170 180 ALKSGATLAL ANKESLIVGG SLVKAAARDG QIVPVDSEHS AIAQCLRSGS AAEVDKLVLT 190 200 210 220 230 240 ASGGPFRGRS GEELHGVTPQ EALAHPTWDM GLMVTTNSAT LVNKGLEVIE AHLLFDIPLD 250 260 270 280 290 300 RIDVVVHPQS VVHSMVQFVD GSTIAQASPP DMRLPIALGL GWPGRVPNAA APCDWTKAAT 310 320 330 340 350 360 WTFEPLDATA FPAVDLAKDA AKQGSTFPAV FNAANEEAVT AFHGGRIRFT DIVDTVDAVL 370 380 390 SEHTGSSRLT VESVLDAESW ARARAHERLA VSSL

References

[1]

"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001341 Genomic DNA. Translation: ACL39401.1.
RefSeq	YP_002487490.1. NC_011886.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8HFR2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7292860.
GenomeReviews	Gene locus Achl_1411 in contig CP001341_GR.
KEGG	ach:Achl_1411.
PATRIC	20989357. VBIArtChl38304_2073.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG430762.
OMA	IHSMVEY.
ProtClustDB	PRK05447.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_ARTCA

Accession

Primary (citable) accession number: B8HFR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program