Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B8HF09 (ISPE_ARTCA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Ordered Locus Names:Achl_1284
OrganismArthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]
Taxonomic identifier452863 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3203204-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061
PRO_1000117876

Regions

Nucleotide binding112 – 12211ATP Potential

Sites

Active site201 By similarity
Active site1541 By similarity

Sequences

Sequence LengthMass (Da)Tools
B8HF09 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: DE557EC170B2EE35

FASTA32032,634
        10         20         30         40         50         60 
MNTPAVRFAA RTVRVKAPGK INVSLDVGPR RPDGYHSVAS VYLAVSLYEE VAATSTETPG 

        70         80         90        100        110        120 
ITVSLSPDST LDLDAVDIPL DASNLAYRAA AIMADVSEHA TGVHLEITKR VPVAGGMGGG 

       130        140        150        160        170        180 
SADAAATLLA CDALWNSGLS REELAHLAAE LGADVPFSLL GGTAVGLGVG DQLSPALAKA 

       190        200        210        220        230        240 
KTHWVLVTAD FGLSTPEVFR TLDRLREAEG VDADEPTGVD PNILAALRGG DADALSRILV 

       250        260        270        280        290        300 
NDLQRASIEL APSLRDTLGI GESHGAIAGI VSGSGPTVAL LADDSVAAEA LAEDLQHQGL 

       310        320 
SALAVHGPVP GARIISDTLL 

« Hide

References

[1]"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001341 Genomic DNA. Translation: ACL39275.1.
RefSeqYP_002487364.1. NC_011886.1.

3D structure databases

ProteinModelPortalB8HF09.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING452863.Achl_1284.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL39275; ACL39275; Achl_1284.
GeneID7292730.
KEGGach:Achl_1284.
PATRIC20989089. VBIArtChl38304_1942.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1947.
HOGENOMHOG000019600.
KOK00919.
OMAEGYHPIS.
OrthoDBEOG62VNQ2.

Enzyme and pathway databases

BioCycACHL452863:GH1A-1307-MONOMER.
UniPathwayUPA00056; UER00094.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPMF_00061. IspE.
InterProIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsTIGR00154. ispE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_ARTCA
AccessionPrimary (citable) accession number: B8HF09
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways