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B8HDH4

- BIOB_ARTCA

UniProt

B8HDH4 - BIOB_ARTCA

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Protein
Biotin synthase
Gene
bioB, Achl_0984
Organism
Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi116 – 1161Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi149 – 1491Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi208 – 2081Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi278 – 2781Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciACHL452863:GH1A-1003-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Achl_0984
OrganismiArthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360)
Taxonomic identifieri452863 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
ProteomesiUP000002505: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Biotin synthaseUniRule annotation
PRO_0000381209Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi452863.Achl_0984.

Structurei

3D structure databases

ProteinModelPortaliB8HDH4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B8HDH4-1 [UniParc]FASTAAdd to Basket

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MTPTVHTWPI LETARTQVLE QGIGLTEDQL VEVLQLPDEA LPQALELAHQ    50
VRLRHCGEDV EVEGIVSIKT GGCPEDCHFC SQSGLFDSPV RGVWLDIPEL 100
VKAAKETAAT GATEFCIVAA VRGPDIKLMN QVKFAIGRIN EEVDINIACS 150
LGMLTQQQVD QLAGWGVHRY NHNLETARSF FPQVVTTHTY EERLETCAMV 200
KAAGMELCCG ALLGMGESLH QRAELAAQLA ALEPHEVPLN FLNPRPGTPL 250
ENQGLMDGKD ALRAIAAFRL AMPRTVLRYA GGRELTLGDL GTREGLLGGI 300
NAVIVGNYLT TLGRPATADL SLLVDLNMPI RELQKTL 337
Length:337
Mass (Da):36,615
Last modified:March 3, 2009 - v1
Checksum:iF941DCAF3950985A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001341 Genomic DNA. Translation: ACL38979.1.
RefSeqiYP_002487068.1. NC_011886.1.

Genome annotation databases

EnsemblBacteriaiACL38979; ACL38979; Achl_0984.
GeneIDi7292426.
KEGGiach:Achl_0984.
PATRICi20988479. VBIArtChl38304_1641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001341 Genomic DNA. Translation: ACL38979.1 .
RefSeqi YP_002487068.1. NC_011886.1.

3D structure databases

ProteinModelPortali B8HDH4.
ModBasei Search...

Protein-protein interaction databases

STRINGi 452863.Achl_0984.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL38979 ; ACL38979 ; Achl_0984 .
GeneIDi 7292426.
KEGGi ach:Achl_0984.
PATRICi 20988479. VBIArtChl38304_1641.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci ACHL452863:GH1A-1003-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Arthrobacter chlorophenolicus A6."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Entry informationi

Entry nameiBIOB_ARTCA
AccessioniPrimary (citable) accession number: B8HDH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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