B8HCG5 (B8HCG5_ARTCA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

Achl_2616

Organism

Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]

Taxonomic identifier

452863 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	258	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	295	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	391	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	B8HCG5 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: D2BFB901AF2ABD63	FASTA	589	64,185
	10 20 30 40 50 60 MTLVNQGPER FDVWAPDASS VILVADGREY PMVKKDTAPG TEGWWTAPDA PADGEVDYGY 70 80 90 100 110 120 RLDGDSHPLP DPRSRRVPAG VHDLSRTFDP AAYAWQDSAW KGRKLTGSVI YELHVGTFTP 130 140 150 160 170 180 EGTLDAAAGK LGYLVDLGID FVELLPVNGF NGTHNWGYDG VQWYAVHEGY GGPAAYQRFV 190 200 210 220 230 240 DAAHSAGLGV IQDVVYNHLG PSGNYLPKFG PYLKQGDANT WGDSVNLDGE GSDVVREYIL 250 260 270 280 290 300 DNLALWLRDY HVDGLRLDAV HALRDERAVH ILEELGALGD AVSAETGLPK TLIAESDLNN 310 320 330 340 350 360 PRLIYPRDAN GYGLEGQWSD DFHHAVHVSV SGETTGYYSD FESLAVLAKV LKDGFLHDGS 370 380 390 400 410 420 YSSFRGRHHG RPINASLVTP AALVVCNQNH DQIGNRATGD RLSQSLSYGQ LAVAAVLTLT 430 440 450 460 470 480 SPFTPMLFMG EEYGATTPWQ FFTSHAEPEL GKATAEGRIK EFERMGWDPA VVPDPQDPET 490 500 510 520 530 540 FRRSKLNWDE AAMGDHARLL ELYKSLTALR REHPELAGLG FGETGVAFDD DSGWLRFRRG 550 560 570 580 SVEVLVNFAD AKVRLDSAGG TLLLSTDEGT SQDGDALALA PWSAAIVKS

References

[1]

"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001341 Genomic DNA. Translation: ACL40581.1.
RefSeq	YP_002488670.1. NC_011886.1.
3D structure databases
ProteinModelPortal	B8HCG5.
ModBase	Search...
Protein-protein interaction databases
STRING	B8HCG5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7294092.
GenomeReviews	Gene locus Achl_2616 in contig CP001341_GR.
KEGG	ach:Achl_2616.
PATRIC	20991867. VBIArtChl38304_3301.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK849609.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02922. CBM_48. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8HCG5_ARTCA

Accession

Primary (citable) accession number: B8HCG5

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)