B8HCC5 (B8HCC5_ARTCA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344
EC=6.3.5.2 HAMAP MF_00344

Alternative name(s):
GMP synthetase HAMAP MF_00344
Glutamine amidotransferase HAMAP MF_00344

Gene names

Name:	guaA HAMAP MF_00344
Ordered Locus Names:	Achl_2576

Organism

Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]

Taxonomic identifier

452863 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 GMP-binding domain. HAMAP MF_00344 Contains 1 glutamine amidotransferase type-1 domain. HAMAP MF_00344

Ontologies

Keywords
Biological process	GMP biosynthesis HAMAP MF_00344 Purine biosynthesis
Domain	Glutamine amidotransferase HAMAP MF_00344
Ligand	ATP-binding HAMAP MF_00344 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00344
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	13 – 204	192	Glutamine amidotransferase type-1 By similarity HAMAP MF_00344
	Domain	236 – 404	169	GMP-binding By similarity HAMAP MF_00344
	Nucleotide binding	232 – 238	7	ATP By similarity HAMAP MF_00344
Sites
	Active site	90	1	Nucleophile By similarity HAMAP MF_00344
	Active site	178	1	By similarity HAMAP MF_00344
	Active site	180	1	By similarity HAMAP MF_00344

Sequences

	Sequence		Length	Mass (Da)
	B8HCC5 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: DF656885AA805D49	FASTA	529	56,912
	10 20 30 40 50 60 MTTPTASQTS QKPVLVVDYG AQYAQLIARR VREANVYSEV VPHTYTTEQL LAKNPAAIIL 70 80 90 100 110 120 SGGPSSVYAD GAPSVGADLF EAGIPVFGIC YGFQAMANAL GGKVDKTGLR EYGSTQTTIL 130 140 150 160 170 180 GEGRSVLEGM PQHQNTWMSH GDSVHAAPEG FEVLATTAGA EVAAFANEEK CLYGVQWHPE 190 200 210 220 230 240 VKHSAYGQQV LENFLFKGAK IEPNWTTGNI LEEQVERIRN QIGDARVICG LSGGVDSAVA 250 260 270 280 290 300 AALVQRAVGD QLTCVFVDHG LLREGEAEQV ERDFVAATGV KLYIANEQER FLSALAGVSD 310 320 330 340 350 360 PETKRKIIGR EFIRAFEEAE LAIIAEAAAH GEKIKFLVQG TLYPDVVESG GGEGAANIKS 370 380 390 400 410 420 HHNVGGLPED LQFELVEPLR ALFKDEVRAV GAQLGLPQEI VGRQPFPGPG LGIRIVGDVT 430 440 450 460 470 480 KERLDLLRKA DAIARAELTA AGLDNEVWQM PVVLLADVRS VGVMGDGRTY GHPIVLRPVS 490 500 510 520 SEDAMTADWS RLPYDLLARI SNRITNEVDG VNRVVLDVTS KPPGTIEWE

References

[1]

"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001341 Genomic DNA. Translation: ACL40541.1.
RefSeq	YP_002488630.1. NC_011886.1.
3D structure databases
ProteinModelPortal	B8HCC5.
ModBase	Search...
Protein-protein interaction databases
STRING	B8HCC5.
Protein family/group databases
MEROPS	C26.A17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7294052.
GenomeReviews	Gene locus Achl_2576 in contig CP001341_GR.
KEGG	ach:Achl_2576.
PATRIC	20991791. VBIArtChl38304_3263.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	DGRTYEY.
ProtClustDB	PRK00074.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8HCC5_ARTCA

Accession

Primary (citable) accession number: B8HCC5

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)