B8HBR2 (GSA_ARTCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	Achl_2485

Organism

Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]

Taxonomic identifier

452863 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter › Arthrobacter chlorophenolicus

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_0000382254
Amino acid modifications
	Modified residue	277	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8HBR2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: BC563DBE004CFA83	FASTA	443	45,761
	10 20 30 40 50 60 MTSSTPRNEE LFDRARQLMP GGVNSPVRAF GSVGGTPRFM VSAKGPYLMD ADGNEYVDLV 70 80 90 100 110 120 CSWGPALLGH AHPAVLDAVH AAVDRGLSFG ASTPDEANLA EIVMERVPAV ERLRMVSTGT 130 140 150 160 170 180 EATMTAVRLA RGFTGRNLII KFAGCYHGHL DGLLAAAGSG VATLALPGSA GVTEATAAET 190 200 210 220 230 240 LVLPYNDLAA VKEAFATHGS NIAAVITEAA PANMGVVTPG EGFNLGLSRI TREHGALLIV 250 260 270 280 290 300 DEVLTGFRTG YSGYWGLTGG APDATDPWTP DLLTFGKVIG GGMPTAALGG RADVMDYLAP 310 320 330 340 350 360 TGPVYQAGTL SGNPVAMAAG VATLTHATRD VYSYIDVRSL ELSSALSSAL DAAGVDHSIQ 370 380 390 400 410 420 FAGNLFSVAF GTSAHGVHNY ADAQGQEAFR YAPFFHSMLE SGVYLPPSVF EAWFLSAAHD 430 440 DAAMNRIFDA LPAAAKAAAA AQG

References

[1]

"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001341 Genomic DNA. Translation: ACL40450.1.
RefSeq	YP_002488539.1. NC_011886.1.
3D structure databases
ProteinModelPortal	B8HBR2.
ModBase	Search...
Protein-protein interaction databases
STRING	452863.Achl_2485.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL40450; ACL40450; Achl_2485.
GeneID	7293960.
KEGG	ach:Achl_2485.
PATRIC	20991607. VBIArtChl38304_3172.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
OMA	FGHADEE.
ProtClustDB	PRK00062.
Enzyme and pathway databases
BioCyc	ACHL452863:GH1A-2537-MONOMER.
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_ARTCA

Accession

Primary (citable) accession number: B8HBR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program