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B8HAC9 (LEUC_ARTCA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase large subunit

EC=4.2.1.33
Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase
Gene names
Name:leuC
Ordered Locus Names:Achl_2256
OrganismArthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]
Taxonomic identifier452863 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01026

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01026

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01026

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01026

Subunit structure

Heterodimer of LeuC and LeuD By similarity. HAMAP-Rule MF_01026

Sequence similarities

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4844843-isopropylmalate dehydratase large subunit HAMAP-Rule MF_01026
PRO_1000149349

Sites

Metal binding3521Iron-sulfur (4Fe-4S) By similarity
Metal binding4121Iron-sulfur (4Fe-4S) By similarity
Metal binding4151Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B8HAC9 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 1E8C7C9A40FE5C82

FASTA48451,545
        10         20         30         40         50         60 
MAKTLAEKVW DAHVVRKGDG AGANAQPDLL FIDLHLVHEV TSPQAFEGLR LAGRPLRRPD 

        70         80         90        100        110        120 
LTIATEDHNT PTLDIDKPIA DLTSRTQIQT LRNNCAEFGV RLHSLGDAEQ GIVHVVGPQL 

       130        140        150        160        170        180 
GLTQPGMTVV CGDSHTSTHG AFGALAMGIG TSEVEHVMAT QTLSLKPFKT MAINVEGTLR 

       190        200        210        220        230        240 
PGVTAKDIIL AVIAKIGTGG GQGYVLEYRG SAIRALSMDA RMTICNMSIE AGARAGMVAP 

       250        260        270        280        290        300 
DETTYAYMQG RPHAPEGADW DAAVEYWNTL KTDDDATFDV EVDLDADTLE PFVTWGTNPG 

       310        320        330        340        350        360 
QGVSLSQAVP SPEDFGDENA KAAAERALQY MGLEAGTPMK DIRVDTVFLG SCTNSRIEDL 

       370        380        390        400        410        420 
RAAADIIRGR EKDPKVRMLV VPGSARVRLE AEAEGLDRVF KDFGAEWRFA GCSMCLGMNP 

       430        440        450        460        470        480 
DQLEPGERCA STSNRNFEGR QGKGGRTHLV SPVVAAATAV RGTLSSPSDL DPAPASAAIR 


TDAA 

« Hide

References

[1]"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001341 Genomic DNA. Translation: ACL40221.1.
RefSeqYP_002488310.1. NC_011886.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING452863.Achl_2256.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL40221; ACL40221; Achl_2256.
GeneID7293724.
KEGGach:Achl_2256.
PATRIC20991127. VBIArtChl38304_2939.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0065.
HOGENOMHOG000226972.
KOK01703.
OMAQARAKTM.
OrthoDBEOG600DP5.

Enzyme and pathway databases

BioCycACHL452863:GH1A-2301-MONOMER.
UniPathwayUPA00048; UER00071.

Family and domain databases

Gene3D3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPMF_01026. LeuC_type1.
InterProIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PfamPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF53732. SSF53732. 1 hit.
TIGRFAMsTIGR00170. leuC. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEUC_ARTCA
AccessionPrimary (citable) accession number: B8HAC9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways