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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Pseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6) (Arthrobacter chlorophenolicus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. Branched-chain-amino-acid aminotransferase (Achl_2267)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi352Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi412Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi415Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:Achl_2256
OrganismiPseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6) (Arthrobacter chlorophenolicus)
Taxonomic identifieri452863 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaePseudarthrobacter
Proteomesi
  • UP000002505 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001493491 – 4843-isopropylmalate dehydratase large subunitAdd BLAST484

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi452863.Achl_2256.

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQI. Bacteria.
COG0065. LUCA.
HOGENOMiHOG000226972.
KOiK01703.
OrthoDBiPOG091H02GD.

Family and domain databases

CDDicd01583. IPMI. 1 hit.
Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1. 1 hit.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR033941. IPMI_cat.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 2 hits.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8HAC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTLAEKVW DAHVVRKGDG AGANAQPDLL FIDLHLVHEV TSPQAFEGLR
60 70 80 90 100
LAGRPLRRPD LTIATEDHNT PTLDIDKPIA DLTSRTQIQT LRNNCAEFGV
110 120 130 140 150
RLHSLGDAEQ GIVHVVGPQL GLTQPGMTVV CGDSHTSTHG AFGALAMGIG
160 170 180 190 200
TSEVEHVMAT QTLSLKPFKT MAINVEGTLR PGVTAKDIIL AVIAKIGTGG
210 220 230 240 250
GQGYVLEYRG SAIRALSMDA RMTICNMSIE AGARAGMVAP DETTYAYMQG
260 270 280 290 300
RPHAPEGADW DAAVEYWNTL KTDDDATFDV EVDLDADTLE PFVTWGTNPG
310 320 330 340 350
QGVSLSQAVP SPEDFGDENA KAAAERALQY MGLEAGTPMK DIRVDTVFLG
360 370 380 390 400
SCTNSRIEDL RAAADIIRGR EKDPKVRMLV VPGSARVRLE AEAEGLDRVF
410 420 430 440 450
KDFGAEWRFA GCSMCLGMNP DQLEPGERCA STSNRNFEGR QGKGGRTHLV
460 470 480
SPVVAAATAV RGTLSSPSDL DPAPASAAIR TDAA
Length:484
Mass (Da):51,545
Last modified:March 3, 2009 - v1
Checksum:i1E8C7C9A40FE5C82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001341 Genomic DNA. Translation: ACL40221.1.
RefSeqiWP_015937436.1. NC_011886.1.

Genome annotation databases

EnsemblBacteriaiACL40221; ACL40221; Achl_2256.
KEGGiach:Achl_2256.
PATRICi20991127. VBIArtChl38304_2939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001341 Genomic DNA. Translation: ACL40221.1.
RefSeqiWP_015937436.1. NC_011886.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi452863.Achl_2256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL40221; ACL40221; Achl_2256.
KEGGiach:Achl_2256.
PATRICi20991127. VBIArtChl38304_2939.

Phylogenomic databases

eggNOGiENOG4105CQI. Bacteria.
COG0065. LUCA.
HOGENOMiHOG000226972.
KOiK01703.
OrthoDBiPOG091H02GD.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Family and domain databases

CDDicd01583. IPMI. 1 hit.
Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1. 1 hit.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR033941. IPMI_cat.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 2 hits.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEUC_PSECP
AccessioniPrimary (citable) accession number: B8HAC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 30, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.