Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Arthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi352 – 3521Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi412 – 4121Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi415 – 4151Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciACHL452863:GH1A-2301-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:Achl_2256
OrganismiArthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360)
Taxonomic identifieri452863 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
ProteomesiUP000002505 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4844843-isopropylmalate dehydratase large subunitPRO_1000149349Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi452863.Achl_2256.

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiDKVWDLH.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8HAC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTLAEKVW DAHVVRKGDG AGANAQPDLL FIDLHLVHEV TSPQAFEGLR
60 70 80 90 100
LAGRPLRRPD LTIATEDHNT PTLDIDKPIA DLTSRTQIQT LRNNCAEFGV
110 120 130 140 150
RLHSLGDAEQ GIVHVVGPQL GLTQPGMTVV CGDSHTSTHG AFGALAMGIG
160 170 180 190 200
TSEVEHVMAT QTLSLKPFKT MAINVEGTLR PGVTAKDIIL AVIAKIGTGG
210 220 230 240 250
GQGYVLEYRG SAIRALSMDA RMTICNMSIE AGARAGMVAP DETTYAYMQG
260 270 280 290 300
RPHAPEGADW DAAVEYWNTL KTDDDATFDV EVDLDADTLE PFVTWGTNPG
310 320 330 340 350
QGVSLSQAVP SPEDFGDENA KAAAERALQY MGLEAGTPMK DIRVDTVFLG
360 370 380 390 400
SCTNSRIEDL RAAADIIRGR EKDPKVRMLV VPGSARVRLE AEAEGLDRVF
410 420 430 440 450
KDFGAEWRFA GCSMCLGMNP DQLEPGERCA STSNRNFEGR QGKGGRTHLV
460 470 480
SPVVAAATAV RGTLSSPSDL DPAPASAAIR TDAA
Length:484
Mass (Da):51,545
Last modified:March 2, 2009 - v1
Checksum:i1E8C7C9A40FE5C82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001341 Genomic DNA. Translation: ACL40221.1.
RefSeqiWP_015937436.1. NC_011886.1.
YP_002488310.1. NC_011886.1.

Genome annotation databases

EnsemblBacteriaiACL40221; ACL40221; Achl_2256.
GeneIDi7293724.
KEGGiach:Achl_2256.
PATRICi20991127. VBIArtChl38304_2939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001341 Genomic DNA. Translation: ACL40221.1.
RefSeqiWP_015937436.1. NC_011886.1.
YP_002488310.1. NC_011886.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi452863.Achl_2256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL40221; ACL40221; Achl_2256.
GeneIDi7293724.
KEGGiach:Achl_2256.
PATRICi20991127. VBIArtChl38304_2939.

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiDKVWDLH.
OrthoDBiEOG600DP5.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.
BioCyciACHL452863:GH1A-2301-MONOMER.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Arthrobacter chlorophenolicus A6."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Entry informationi

Entry nameiLEUC_ARTCA
AccessioniPrimary (citable) accession number: B8HAC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2009
Last sequence update: March 2, 2009
Last modified: February 3, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.