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B8HAC4 (DDL_ARTCA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Achl_2251
OrganismArthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360) [Complete proteome] [HAMAP]
Taxonomic identifier452863 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000117446

Regions

Domain161 – 372212ATP-grasp
Nucleotide binding193 – 24856ATP By similarity

Sites

Metal binding3261Magnesium or manganese 1 By similarity
Metal binding3391Magnesium or manganese 1 By similarity
Metal binding3391Magnesium or manganese 2 By similarity
Metal binding3411Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B8HAC4 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 10D3114381BE1BCE

FASTA38240,523
        10         20         30         40         50         60 
MSHKDLTSGE PAGRPKPRVA VLFGGRSSEH AVSCVTAAGV LGAINKDKYD VIPIGIAKSG 

        70         80         90        100        110        120 
QWVLAGGDTA QWSLAGKALP EVAPSEQTVT LAEIGGEHQL IVAAPNEVPQ ELGTVDVVFP 

       130        140        150        160        170        180 
LLHGPFGEDG TIQGLLELSD TRYVGAGVLA SAVGMDKHFM KVVFESAGLH VGPYVAVTDR 

       190        200        210        220        230        240 
QWQKDPEAVR KQVDRLGFPV FVKPARAGSS MGISKVDSLE GLDAAIEEAR RHDLKLVIEA 

       250        260        270        280        290        300 
GIVGREIECA VLEGRGTDAP RTSLPGEISV AGGGHEFYDF NAKYVEDDAA SLSCPADIPD 

       310        320        330        340        350        360 
EAIARVRELA AAAFDAVGAE GLSRVDFFYT PDGDLIINEI NTMPGFTPKS MYPQMWAKSG 

       370        380 
LGYAELIDEL IHLALNRKTG LR 

« Hide

References

[1]"Complete sequence of chromosome of Arthrobacter chlorophenolicus A6."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Goltsman E., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Jansson J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A6 / ATCC 700700 / DSM 12829 / JCM 12360.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001341 Genomic DNA. Translation: ACL40216.1.
RefSeqYP_002488305.1. NC_011886.1.

3D structure databases

ProteinModelPortalB8HAC4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING452863.Achl_2251.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL40216; ACL40216; Achl_2251.
GeneID7293719.
KEGGach:Achl_2251.
PATRIC20991115. VBIArtChl38304_2933.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycACHL452863:GH1A-2296-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_ARTCA
AccessionPrimary (citable) accession number: B8HAC4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways