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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Pseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6) (Arthrobacter chlorophenolicus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumUniRule annotation1
Metal bindingi70MagnesiumUniRule annotation1
Metal bindingi143MagnesiumUniRule annotation1
Metal bindingi146MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:Achl_2033
OrganismiPseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6) (Arthrobacter chlorophenolicus)
Taxonomic identifieri452863 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaePseudarthrobacter
Proteomesi
  • UP000002505 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001952341 – 192Crossover junction endodeoxyribonuclease RuvCAdd BLAST192

Interactioni

Protein-protein interaction databases

STRINGi452863.Achl_2033

Structurei

3D structure databases

SMRiB8H9D8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012182
KOiK01159
OMAiGLTRCGI
OrthoDBiPOG091H0202

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit

Sequencei

Sequence statusi: Complete.

B8H9D8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLRVLGVDP GLTRCGIGVV DVERNRRATM VAVGVVGTSP DESLDQRLLV
60 70 80 90 100
IATAIDEWLD RYEPQVLAVE RVFSQLNVST VMGVAQASGV VIAAAARRGI
110 120 130 140 150
PVALHTPSEV KAAVTGSGSS NKDAVTKLVT KILRLDAPPR PADAADALAL
160 170 180 190
AITHAWRAGS GASVATTGPG SSSLTPAQRA WAEAEAKARR AR
Length:192
Mass (Da):19,910
Last modified:March 3, 2009 - v1
Checksum:i7E49AA9B9B1400CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001341 Genomic DNA Translation: ACL40007.1
RefSeqiWP_015937225.1, NC_011886.1

Genome annotation databases

EnsemblBacteriaiACL40007; ACL40007; Achl_2033
KEGGiach:Achl_2033

Similar proteinsi

Entry informationi

Entry nameiRUVC_PSECP
AccessioniPrimary (citable) accession number: B8H9D8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 23, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health