B8H510 (ISPE_CAUCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	CCNA_01397

Organism

Caulobacter crescentus (strain NA1000 / CB15N) [Complete proteome] [HAMAP]

Taxonomic identifier

565050 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000190679
Regions
	Nucleotide binding	96 – 106	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	138	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8H510 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 610CBB5EBE7A0B53	FASTA	290	30,307
	10 20 30 40 50 60 MRLSAFAPAK VNLFLHVGGP DGEGYHPISS LMVFADVGDR VNLQPADAPA FETSGPFGDQ 70 80 90 100 110 120 IPAGGDNLVV RAGQAFHRRL GGPVPPYRLI LEKHLPIAAG LGGGSSDAGA ALKLLRDALA 130 140 150 160 170 180 PALSDDDLEA LAASLGADGA ACLRARALIA EGRGERLSPA PRLPELNAVL VNPGAPSPTG 190 200 210 220 230 240 AVYRAYDAGV HPDGAAMPPM PDHLESAEEA AAWLAFATRN DLEAPAVRLE PHIGEVLDVL 250 260 270 280 290 RGEPESLLVR MSGSGATCFA LCASDIEAEG LAERLETMRP DWWVRRCRLS

References

[1]

"The genetic basis of laboratory adaptation in Caulobacter crescentus." Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S. J. Bacteriol. 192:3678-3688(2010) [PubMed: 20472802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NA1000 / CB15N.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001340 Genomic DNA. Translation: ACL94862.1.
RefSeq	YP_002516770.1. NC_011916.1.
3D structure databases
ProteinModelPortal	B8H510.
ModBase	Search...
Protein-protein interaction databases
STRING	B8H510.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7331855.
GenomeReviews	Gene locus CCNA_01397 in contig CP001340_GR.
KEGG	ccs:CCNA_01397.
PATRIC	21307367. VBICauCre52860_1383.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CQLARMS.
ProtClustDB	PRK14608.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CAUCN

Accession

Primary (citable) accession number: B8H510

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program