Reviewed, UniProtKB/Swiss-Prot B8H427 (DNAE2_CAUCN)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	CCNA_03317

Organism

Caulobacter crescentus (strain NA1000 / CB15N) [Complete proteome] [HAMAP]

Taxonomic identifier

565050 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	1083 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). Along with imuA and imuB is required for the error-prone processing of DNA lesions. Ref.2
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Induction	Up-regulated in response to DNA damage in a recA-dependent manner. HAMAP MF_01902
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1083	1083	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000378287

Sequences

	Sequence		Length	Mass (Da)
	B8H427-1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 124E5F216A555E64	FASTA	1,083	120,800
	10 20 30 40 50 60 MRPPVYAELQ ATTNFSFLRG ASHAEELALT AEALGLTAIG IVDRNSLAGV VRAWTAAKRR 70 80 90 100 110 120 SIRVLTGCRL DFMDGAPSLL CYPTDREAFG RLTRLLTIGQ LRAEKGECHL TWRDFLDHSE 130 140 150 160 170 180 GQLGLIVPPR VLDDSFEQHL TRMAGDLRGR SWLAASRAYA ARDLQRLARL ESLGRTSGAP 190 200 210 220 230 240 IVATNDVLYH GPERRPLQDV ISCVREHCAI QEAGFRLEAN AERHIKSPEE MARLFDRWPR 250 260 270 280 290 300 AVERTVEIVE RIGFDLKDIR EQYPDEPVPP GKTAMQHLTD LTWKGAAWRY PNGVSPKVTA 310 320 330 340 350 360 QIQEELRLIE KMDYPNYFIT VHDIVREARS MGILCQGRGS AANSSVCFCL GVTAIDPTEH 370 380 390 400 410 420 RLLFTRFISE NRGEPPDIDV DFEHDRREEV MQYVFQRYGR AYAAICGTVI HYRPRSAIRD 430 440 450 460 470 480 VGKALGLTED VTSLLAGTVW GSWGDGLPED HLRNAGLDPK APEIARAVGL ANDLIGFPRH 490 500 510 520 530 540 LSQHVGGFVL TKRRLDETVP IGKAAMKDRT FIEWDKDDID SLGLMKVDIL ALGMLHAIQR 550 560 570 580 590 600 AMTMLREDHG QDWLKDLADI PKEVPGVYDM LCAADSVGVF QVESRAQMSM LPRLRPREFY 610 620 630 640 650 660 DLVIQVAIVR PGPIQGDMVH PYLKRRNKEE PVDWPKPSPE HGPPDELQEI LGKTFGVPLF 670 680 690 700 710 720 QEQAMSLAIE AAKFTPDEAD GLRKAMATFR NLGSPDAYRN KFIEGMVGRG YERAFAERCF 730 740 750 760 770 780 KQIEGFSHYG FPESHAASFA KLVYVSAWIK WAWPDVFCAA LINAQPMGFY QPAQLVRDAR 790 800 810 820 830 840 EHGVEVLPPD ILTSDWDCTL APISTGFRPP RVRHDKVACQ ETRPRWKAVR LGFRQIKGLR 850 860 870 880 890 900 ETLDIPPLLK ARGEGARTPA EFAQGGVPQK ALELLAEADA FASVGLSRRE ALWAVKGLKG 910 920 930 940 950 960 EHKAPVQAPL LAGLPLFEER VALPAMAATQ EVAEDYRTTS LSLKAHPIGF YRSMLAARGV 970 980 990 1000 1010 1020 VPAERLLSLK DGARVSVAGL VLIRQRPGTA KGVVFVTLED ETGVANAVVW KDRFDAARNV 1030 1040 1050 1060 1070 1080 VMTASFLIVH GRVQRADNVI HVVAERFTDL SAELSSLRDE PGAPAPRIRQ KVSGRLLRSR DFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative genome analysis of Caulobacter crescentus strains CB15 and NA1000." Marks M.E., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"An SOS-regulated operon involved in damage-inducible mutagenesis in Caulobacter crescentus." Galhardo R.S., Rocha R.P., Marques M.V., Menck C.F.M. Nucleic Acids Res. 33:2603-2614(2005) [PubMed: 15886391] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001340 Genomic DNA. Translation: ACL96781.1.
RefSeq	YP_002518689.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7330340.
GenomeReviews	Gene locus CCNA_03317 in contig CP001340_GR.
KEGG	ccs:CCNA_03317.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_CAUCN

Accession

Primary (citable) accession number: B8H427

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	March 3, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)