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Protein

Imidazolonepropionase

Gene

hutI

Organism
Caulobacter crescentus (strain NA1000 / CB15N)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+.UniRule annotation

Cofactori

Zn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 1 zinc or iron ion per subunit.UniRule annotation

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 3 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Zinc or ironUniRule annotation
Metal bindingi72 – 721Zinc or ironUniRule annotation
Binding sitei79 – 791SubstrateUniRule annotation
Binding sitei92 – 921SubstrateUniRule annotation
Binding sitei142 – 1421SubstrateUniRule annotation
Binding sitei175 – 1751SubstrateUniRule annotation
Metal bindingi240 – 2401Zinc or ironUniRule annotation
Binding sitei243 – 2431SubstrateUniRule annotation
Metal bindingi315 – 3151Zinc or ironUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Histidine metabolism

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCAULONA1000:CCNA_01011-MONOMER.
UniPathwayiUPA00379; UER00551.

Protein family/group databases

MEROPSiM38.980.

Names & Taxonomyi

Protein namesi
Recommended name:
ImidazolonepropionaseUniRule annotation (EC:3.5.2.7UniRule annotation)
Alternative name(s):
Imidazolone-5-propionate hydrolaseUniRule annotation
Gene namesi
Name:hutIUniRule annotation
Ordered Locus Names:CCNA_01011
OrganismiCaulobacter crescentus (strain NA1000 / CB15N)
Taxonomic identifieri565050 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001364 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401ImidazolonepropionasePRO_1000133882Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB8H2S2.
SMRiB8H2S2. Positions 6-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HutI family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218461.
KOiK01468.
OMAiDHCTHLT.
OrthoDBiEOG63C0XD.

Family and domain databases

Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_00372. HutI.
InterProiIPR006680. Amidohydro-rel.
IPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01224. hutI. 1 hit.

Sequencei

Sequence statusi: Complete.

B8H2S2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCDRVWINA RLATLAPGRQ GLGIVEDGVV ACLGGRIAYA GPAAAAPTFE
60 70 80 90 100
AAESIDVDGR WITPGLIDPH THLVFAGDRA HEFELRLAGA SYEEIARAGG
110 120 130 140 150
GIVSTMRATR AASEAELIAA ALPRLDALLA EGVTTVEIKS GYGLSLDDEL
160 170 180 190 200
KSLRAARALA DIRRVGVTTT FLGAHALPPE YRDDEDGYVD LVCQTMIPAI
210 220 230 240 250
AARGLADAVD GFCEGIGFSP AQIRRVFDAA KAHGLPVKLH AEQLSNLSGA
260 270 280 290 300
ALAAEYNALS ADHLEHLDAA GIAAMAASGT VATLLPGAYY FTRETLVPPI
310 320 330 340 350
AALRAAGVPM ALATDCNPGT SPLTSPLLVM NMAATLFRMT VEECLAGVTR
360 370 380 390 400
EAARALGLLA DRGTLEAGKA CDLAIWDVER PAELVYRMGF NPLHARVWRG

L
Length:401
Mass (Da):42,114
Last modified:March 3, 2009 - v1
Checksum:iEB5FD3FC64B14A84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001340 Genomic DNA. Translation: ACL94476.1.
RefSeqiWP_010918844.1. NC_011916.1.
YP_002516384.1. NC_011916.1.

Genome annotation databases

EnsemblBacteriaiACL94476; ACL94476; CCNA_01011.
GeneIDi7329759.
KEGGiccs:CCNA_01011.
PATRICi21306567. VBICauCre52860_0993.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001340 Genomic DNA. Translation: ACL94476.1.
RefSeqiWP_010918844.1. NC_011916.1.
YP_002516384.1. NC_011916.1.

3D structure databases

ProteinModelPortaliB8H2S2.
SMRiB8H2S2. Positions 6-400.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM38.980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL94476; ACL94476; CCNA_01011.
GeneIDi7329759.
KEGGiccs:CCNA_01011.
PATRICi21306567. VBICauCre52860_0993.

Phylogenomic databases

HOGENOMiHOG000218461.
KOiK01468.
OMAiDHCTHLT.
OrthoDBiEOG63C0XD.

Enzyme and pathway databases

UniPathwayiUPA00379; UER00551.
BioCyciCAULONA1000:CCNA_01011-MONOMER.

Family and domain databases

Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_00372. HutI.
InterProiIPR006680. Amidohydro-rel.
IPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01224. hutI. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genetic basis of laboratory adaptation in Caulobacter crescentus."
    Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S.
    J. Bacteriol. 192:3678-3688(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NA1000 / CB15N.

Entry informationi

Entry nameiHUTI_CAUCN
AccessioniPrimary (citable) accession number: B8H2S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 11, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.