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B8H040

- B8H040_CAUCN

UniProt

B8H040 - B8H040_CAUCN

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Protein

Phosphoribosylformylglycinamidine synthase subunit PurQ

Gene
purQ, CCNA_02583
Organism
Caulobacter crescentus (strain NA1000 / CB15N)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL By similarity.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotations
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Nucleophile By similarityUniRule annotation
Active sitei195 – 1951 By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation, LigaseUniRule annotationSAAS annotationsImported

Keywords - Biological processi

Purine biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciCAULONA1000:CCNA_02583-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurQUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit I
Glutaminase PurQ
Phosphoribosylformylglycinamidine synthase subunit I
Gene namesi
Name:purQUniRule annotation
Ordered Locus Names:CCNA_02583Imported
OrganismiCaulobacter crescentus (strain NA1000 / CB15N)Imported
Taxonomic identifieri565050 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
ProteomesiUP000001364: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Heterodimer of two subunits, PurQ and PurL By similarity.SAAS annotations
Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi190650.CC_2497.

Structurei

3D structure databases

ProteinModelPortaliB8H040.
SMRiB8H040. Positions 2-217.

Family & Domainsi

Sequence similaritiesi

Contains glutamine amidotransferase type-1 domain.SAAS annotations
Contains glutamine amidotransferase type-domain.SAAS annotations

Keywords - Domaini

Glutamine amidotransferaseUniRule annotationSAAS annotations

Phylogenomic databases

eggNOGiCOG0047.
HOGENOMiHOG000238240.
KOiK01952.
OMAiKFICRPV.
OrthoDBiEOG6P5ZJP.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00421. PurQ.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010075. PRibForGlyAmidine_synth_I.
[Graphical view]
PIRSFiPIRSF001586. FGAM_synth_I. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01737. FGAM_synth_I. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8H040-1 [UniParc]FASTAAdd to Basket

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MKAAVIVFPG SNCDRDCKVA IERSAGARVE MVWHQETALP DDLDLIVLPG    50
GFSYGDYLRC GAMAAQSPVM KEVVSAAGKG VAVVGICNGF QVLTEVGLLP 100
GALLRNAGLK YVCKPVELDI VNGQTRFTAG YGEQRQAVMT VGNGEGNYFA 150
DEETLDRLEG EGQVVFRYKE NPNGSARDIA GIVNKGGNVL GLMPHPDRAF 200
DADLGSEDGA VLFRSIFQSA 220
Length:220
Mass (Da):23,388
Last modified:March 3, 2009 - v1
Checksum:i0041310F86284C0C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001340 Genomic DNA. Translation: ACL96048.1.
RefSeqiWP_010920354.1. NC_011916.1.
YP_002517956.1. NC_011916.1.

Genome annotation databases

EnsemblBacteriaiACL96048; ACL96048; CCNA_02583.
GeneIDi7332933.
KEGGiccs:CCNA_02583.
PATRICi21309709. VBICauCre52860_2533.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001340 Genomic DNA. Translation: ACL96048.1 .
RefSeqi WP_010920354.1. NC_011916.1.
YP_002517956.1. NC_011916.1.

3D structure databases

ProteinModelPortali B8H040.
SMRi B8H040. Positions 2-217.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190650.CC_2497.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL96048 ; ACL96048 ; CCNA_02583 .
GeneIDi 7332933.
KEGGi ccs:CCNA_02583.
PATRICi 21309709. VBICauCre52860_2533.

Phylogenomic databases

eggNOGi COG0047.
HOGENOMi HOG000238240.
KOi K01952.
OMAi KFICRPV.
OrthoDBi EOG6P5ZJP.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci CAULONA1000:CCNA_02583-MONOMER.

Family and domain databases

Gene3Di 3.40.50.880. 1 hit.
HAMAPi MF_00421. PurQ.
InterProi IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010075. PRibForGlyAmidine_synth_I.
[Graphical view ]
PIRSFi PIRSF001586. FGAM_synth_I. 1 hit.
SUPFAMi SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR01737. FGAM_synth_I. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genetic basis of laboratory adaptation in Caulobacter crescentus."
    Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S.
    J. Bacteriol. 192:3678-3688(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NA1000 / CB15N.

Entry informationi

Entry nameiB8H040_CAUCN
AccessioniPrimary (citable) accession number: B8H040
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS By similarity.UniRule annotation

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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