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B8H040

- B8H040_CAUCN

UniProt

B8H040 - B8H040_CAUCN

Protein

Phosphoribosylformylglycinamidine synthase subunit PurQ

Gene

purQ

Organism
Caulobacter crescentus (strain NA1000 / CB15N)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotation
    L-glutamine + H2O = L-glutamate + NH3.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei87 – 871NucleophileUniRule annotation
    Active sitei195 – 1951UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    HydrolaseUniRule annotationSAAS annotation, LigaseUniRule annotationSAAS annotationImported

    Keywords - Biological processi

    Purine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCAULONA1000:CCNA_02583-MONOMER.
    UniPathwayiUPA00074; UER00128.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine synthase subunit PurQUniRule annotation (EC:6.3.5.3UniRule annotation)
    Short name:
    FGAM synthaseUniRule annotation
    Alternative name(s):
    Formylglycinamide ribonucleotide amidotransferase subunit IUniRule annotation
    Glutaminase PurQUniRule annotation
    Phosphoribosylformylglycinamidine synthase subunit IUniRule annotation
    Gene namesi
    Name:purQUniRule annotation
    Ordered Locus Names:CCNA_02583Imported
    OrganismiCaulobacter crescentus (strain NA1000 / CB15N)Imported
    Taxonomic identifieri565050 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
    ProteomesiUP000001364: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi190650.CC_2497.

    Structurei

    3D structure databases

    ProteinModelPortaliB8H040.
    SMRiB8H040. Positions 2-217.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation
    Contains glutamine amidotransferase type-1 domain.SAAS annotation

    Keywords - Domaini

    Glutamine amidotransferaseUniRule annotationSAAS annotation

    Phylogenomic databases

    eggNOGiCOG0047.
    HOGENOMiHOG000238240.
    KOiK01952.
    OMAiKFICRPV.
    OrthoDBiEOG6P5ZJP.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    HAMAPiMF_00421. PurQ.
    InterProiIPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010075. PRibForGlyAmidine_synth_I.
    [Graphical view]
    PIRSFiPIRSF001586. FGAM_synth_I. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01737. FGAM_synth_I. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8H040-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKAAVIVFPG SNCDRDCKVA IERSAGARVE MVWHQETALP DDLDLIVLPG    50
    GFSYGDYLRC GAMAAQSPVM KEVVSAAGKG VAVVGICNGF QVLTEVGLLP 100
    GALLRNAGLK YVCKPVELDI VNGQTRFTAG YGEQRQAVMT VGNGEGNYFA 150
    DEETLDRLEG EGQVVFRYKE NPNGSARDIA GIVNKGGNVL GLMPHPDRAF 200
    DADLGSEDGA VLFRSIFQSA 220
    Length:220
    Mass (Da):23,388
    Last modified:March 3, 2009 - v1
    Checksum:i0041310F86284C0C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001340 Genomic DNA. Translation: ACL96048.1.
    RefSeqiWP_010920354.1. NC_011916.1.
    YP_002517956.1. NC_011916.1.

    Genome annotation databases

    EnsemblBacteriaiACL96048; ACL96048; CCNA_02583.
    GeneIDi7332933.
    KEGGiccs:CCNA_02583.
    PATRICi21309709. VBICauCre52860_2533.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001340 Genomic DNA. Translation: ACL96048.1 .
    RefSeqi WP_010920354.1. NC_011916.1.
    YP_002517956.1. NC_011916.1.

    3D structure databases

    ProteinModelPortali B8H040.
    SMRi B8H040. Positions 2-217.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 190650.CC_2497.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL96048 ; ACL96048 ; CCNA_02583 .
    GeneIDi 7332933.
    KEGGi ccs:CCNA_02583.
    PATRICi 21309709. VBICauCre52860_2533.

    Phylogenomic databases

    eggNOGi COG0047.
    HOGENOMi HOG000238240.
    KOi K01952.
    OMAi KFICRPV.
    OrthoDBi EOG6P5ZJP.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00128 .
    BioCyci CAULONA1000:CCNA_02583-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.880. 1 hit.
    HAMAPi MF_00421. PurQ.
    InterProi IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010075. PRibForGlyAmidine_synth_I.
    [Graphical view ]
    PIRSFi PIRSF001586. FGAM_synth_I. 1 hit.
    SUPFAMi SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR01737. FGAM_synth_I. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genetic basis of laboratory adaptation in Caulobacter crescentus."
      Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S.
      J. Bacteriol. 192:3678-3688(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NA1000 / CB15NImported.

    Entry informationi

    Entry nameiB8H040_CAUCN
    AccessioniPrimary (citable) accession number: B8H040
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 3, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3