Reviewed, UniProtKB/Swiss-Prot B8GZ33 (MTC1_CAUCN)

Names and origin

Protein names

Recommended name:
    Modification methylase CcrMI
      Short name=M.CcrMI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase CcrMI

Gene names

Name:	ccrMIM
Synonyms:	ccrM
Ordered Locus Names:	CCNA_00382

Organism

Caulobacter crescentus (strain NA1000 / CB15N) [Complete proteome] [HAMAP]

Taxonomic identifier

565050 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Modification methylase CcrMI		PRO_0000378305
Experimental info
	Sequence conflict	242	1	Y → D in AAA18913. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	B8GZ33-1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 05F43266F7D4C614	FASTA	358	39,665
	10 20 30 40 50 60 MKFGPETIIH GDCIEQMNAL PEKSVDLIFA DPPYNLQLGG DLLRPDNSKV DAVDDHWDQF 70 80 90 100 110 120 ESFAAYDKFT REWLKAARRV LKDDGAIWVI GSYHNIFRVG VAVQDLGFWI LNDIVWRKSN 130 140 150 160 170 180 PMPNFKGTRF ANAHETLIWA SKSQNAKRYT FNYDALKMAN DEVQMRSDWT IPLCTGEERI 190 200 210 220 230 240 KGADGQKAHP TQKPEALLYR VILSTTKPGD VILDPFFGVG TTGAAAKRLG RKFIGIEREA 250 260 270 280 290 300 EYLEHAKARI AKVVPIAPED LDVMGSKRAE PRVPFGTIVE AGLLSPGDTL YCSKGTHVAK 310 320 330 340 350 VRPDGSITVG DLSGSIHKIG ALVQSAPACN GWTYWHFKTD AGLAPIDVLR AQVRAGMN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Caulobacter DNA methyltransferase that functions only in the predivisional cell." Zweiger G., Marczynski G., Shapiro L. J. Mol. Biol. 235:472-485(1994) [PubMed: 8289276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Comparative genome analysis of Caulobacter crescentus strains CB15 and NA1000." Marks M.E., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01032 Unassigned DNA. Translation: AAA18913.1. CP001340 Genomic DNA. Translation: ACL93849.1.
PIR	S43876.
RefSeq	YP_002515757.1.
3D structure databases
SMR	B8GZ33. Positions 5-252.
ModBase	Search...
Genome annotation databases
GeneID	7331081.
GenomeReviews	Gene locus CCNA_00382 in contig CP001340_GR.
KEGG	ccs:CCNA_00382.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG501679.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. MeTrfase_CN4. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTC1_CAUCN

Accession

Primary (citable) accession number: B8GZ33
Secondary accession number(s): Q45971

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	March 3, 2009
Last modified:	January 19, 2010
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)