B8GZ33 (MTC1_CAUCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase CcrMI
Short name=M.CcrMI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase CcrMI

Gene names

Name:	ccrMIM
Synonyms:	ccrM
Ordered Locus Names:	CCNA_00382

Organism

Caulobacter crescentus (strain NA1000 / CB15N) [Complete proteome] [HAMAP]

Taxonomic identifier

565050 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter › Caulobacter crescentus › Caulobacter crescentus (strain ATCC 19089 / CB15)

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Modification methylase CcrMI		PRO_0000378305
Experimental info
	Sequence conflict	242	1	Y → D in AAA18913. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	B8GZ33 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 05F43266F7D4C614	FASTA	358	39,665
	10 20 30 40 50 60 MKFGPETIIH GDCIEQMNAL PEKSVDLIFA DPPYNLQLGG DLLRPDNSKV DAVDDHWDQF 70 80 90 100 110 120 ESFAAYDKFT REWLKAARRV LKDDGAIWVI GSYHNIFRVG VAVQDLGFWI LNDIVWRKSN 130 140 150 160 170 180 PMPNFKGTRF ANAHETLIWA SKSQNAKRYT FNYDALKMAN DEVQMRSDWT IPLCTGEERI 190 200 210 220 230 240 KGADGQKAHP TQKPEALLYR VILSTTKPGD VILDPFFGVG TTGAAAKRLG RKFIGIEREA 250 260 270 280 290 300 EYLEHAKARI AKVVPIAPED LDVMGSKRAE PRVPFGTIVE AGLLSPGDTL YCSKGTHVAK 310 320 330 340 350 VRPDGSITVG DLSGSIHKIG ALVQSAPACN GWTYWHFKTD AGLAPIDVLR AQVRAGMN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Caulobacter DNA methyltransferase that functions only in the predivisional cell." Zweiger G., Marczynski G., Shapiro L. J. Mol. Biol. 235:472-485(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genetic basis of laboratory adaptation in Caulobacter crescentus." Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S. J. Bacteriol. 192:3678-3688(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NA1000 / CB15N.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01032 Unassigned DNA. Translation: AAA18913.1. CP001340 Genomic DNA. Translation: ACL93849.1.
PIR	S43876.
RefSeq	YP_002515757.1. NC_011916.1.
3D structure databases
ProteinModelPortal	B8GZ33.
SMR	B8GZ33. Positions 12-246.
ModBase	Search...
Protein-protein interaction databases
STRING	190650.CC_0378.
Protein family/group databases
REBASE	19987. M.CcrNAORF382P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL93849; ACL93849; CCNA_00382.
GeneID	7331081.
KEGG	ccs:CCNA_00382.
PATRIC	21305331. VBICauCre52860_0381.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0863.
HOGENOM	HOG000125329.
KO	K13581.
OMA	KVHSTQK.
ProtClustDB	CLSK889886.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTC1_CAUCN

Accession

Primary (citable) accession number: B8GZ33
Secondary accession number(s): Q45971

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program