B8GXP2 (RNPH_CAUCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	CCNA_00151

Organism

Caulobacter crescentus (strain NA1000 / CB15N) [Complete proteome] [HAMAP]

Taxonomic identifier

565050 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter › Caulobacter crescentus › Caulobacter crescentus (strain ATCC 19089 / CB15)

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular_function	tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP-Rule MF_00564		PRO_0000378308
Experimental info
	Sequence conflict	25 – 28	4	EGSC → RAL in AAB01514. Ref.1
	Sequence conflict	206	1	K → T in AAB01514. Ref.1
	Sequence conflict	220 – 234	15	LAEKG…ALQRA → AGREGVQRTVRLLARL in AAB01514. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	B8GXP2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 8DC29753AD7EFE58	FASTA	238	25,549
	10 20 30 40 50 60 MRPSERAPDQ LRAVTLETGV NRYAEGSCLI GFGHTKVLVT ATVEENVPGW MRNKGAGWVT 70 80 90 100 110 120 AEYGMLPRAT HTRGRREAAA GKQTGRTQEI QRLIGRSLRA VVDLKALGER QITLDCDVVQ 130 140 150 160 170 180 ADGGTRTAAI TGAWVALRLA TKYLLDEGVL KTDPILGQVA AVSCGVFNGV PVLDLDYEED 190 200 210 220 230 SNAEADSNFV LTGVGDIVEI QATGEKRGFT RAEFESLYGL AEKGINELFA LQRAAIGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a Caulobacter crescentus operon encoding hrcA, involved in negatively regulating heat-inducible transcription, and the chaperone gene grpE." Roberts R.C., Toochinda C., Avedissian M., Baldini R.L., Gomes S.L., Shapiro L. J. Bacteriol. 178:1829-1841(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genetic basis of laboratory adaptation in Caulobacter crescentus." Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S. J. Bacteriol. 192:3678-3688(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NA1000 / CB15N.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33324 Genomic DNA. Translation: AAB01514.1. CP001340 Genomic DNA. Translation: ACL93618.1.
RefSeq	YP_002515526.1. NC_011916.1.
3D structure databases
ProteinModelPortal	B8GXP2.
SMR	B8GXP2. Positions 2-237.
ModBase	Search...
Protein-protein interaction databases
STRING	190650.CC_0152.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL93618; ACL93618; CCNA_00151.
GeneID	7332405.
KEGG	ccs:CCNA_00151.
PATRIC	21304867. VBICauCre52860_0150.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HOG000229516.
KO	K00989.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Family and domain databases
HAMAP	MF_00564. RNase_PH.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_CAUCN

Accession

Primary (citable) accession number: B8GXP2
Secondary accession number(s): P48196

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program