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Protein

Ribonuclease PH

Gene

rph

Organism
Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.UniRule annotation

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86Phosphate (substrate) bindingUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processrRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)
Short name:
RNase PHUniRule annotation
Alternative name(s):
tRNA nucleotidyltransferaseUniRule annotation
Gene namesi
Name:rphUniRule annotation
Ordered Locus Names:CCNA_00151
OrganismiCaulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)
Taxonomic identifieri565050 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001364 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003783081 – 238Ribonuclease PHAdd BLAST238

Interactioni

Subunit structurei

Homohexameric ring arranged as a trimer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB8GXP2
SMRiB8GXP2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 126Phosphate (substrate) bindingUniRule annotation3

Sequence similaritiesi

Belongs to the RNase PH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229516
KOiK00989
OMAiKGKGQGW
OrthoDBiPOG091H03ML

Family and domain databases

CDDicd11362 RNase_PH_bact, 1 hit
Gene3Di3.30.230.70, 1 hit
HAMAPiMF_00564 RNase_PH, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR027408 PNPase/RNase_PH_dom_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR002381 RNase_PH_bac-type
IPR018336 RNase_PH_CS
PfamiView protein in Pfam
PF01138 RNase_PH, 1 hit
PF03725 RNase_PH_C, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55666 SSF55666, 1 hit
TIGRFAMsiTIGR01966 RNasePH, 1 hit
PROSITEiView protein in PROSITE
PS01277 RIBONUCLEASE_PH, 1 hit

Sequencei

Sequence statusi: Complete.

B8GXP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPSERAPDQ LRAVTLETGV NRYAEGSCLI GFGHTKVLVT ATVEENVPGW
60 70 80 90 100
MRNKGAGWVT AEYGMLPRAT HTRGRREAAA GKQTGRTQEI QRLIGRSLRA
110 120 130 140 150
VVDLKALGER QITLDCDVVQ ADGGTRTAAI TGAWVALRLA TKYLLDEGVL
160 170 180 190 200
KTDPILGQVA AVSCGVFNGV PVLDLDYEED SNAEADSNFV LTGVGDIVEI
210 220 230
QATGEKRGFT RAEFESLYGL AEKGINELFA LQRAAIGG
Length:238
Mass (Da):25,549
Last modified:March 3, 2009 - v1
Checksum:i8DC29753AD7EFE58
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25 – 28EGSC → RAL in AAB01514 (PubMed:8606155).Curated4
Sequence conflicti206K → T in AAB01514 (PubMed:8606155).Curated1
Sequence conflicti220 – 234LAEKG…ALQRA → AGREGVQRTVRLLARL in AAB01514 (PubMed:8606155).CuratedAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33324 Genomic DNA Translation: AAB01514.1
CP001340 Genomic DNA Translation: ACL93618.1
RefSeqiWP_010918041.1, NC_011916.1
YP_002515526.1, NC_011916.1

Genome annotation databases

EnsemblBacteriaiACL93618; ACL93618; CCNA_00151
GeneIDi7332405
KEGGiccs:CCNA_00151
PATRICifig|565050.3.peg.150

Similar proteinsi

Entry informationi

Entry nameiRNPH_CAUVN
AccessioniPrimary (citable) accession number: B8GXP2
Secondary accession number(s): P48196
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: March 3, 2009
Last modified: April 25, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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