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B8GX04

- TRMB_CAUCN

UniProt

B8GX04 - TRMB_CAUCN

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Caulobacter crescentus (strain NA1000 / CB15N)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591S-adenosyl-L-methionineUniRule annotation
Binding sitei84 – 841S-adenosyl-L-methionineUniRule annotation
Binding sitei111 – 1111S-adenosyl-L-methionineUniRule annotation
Active sitei133 – 1331By similarity
Binding sitei133 – 1331S-adenosyl-L-methionineUniRule annotation
Binding sitei137 – 1371SubstrateUniRule annotation
Binding sitei169 – 1691SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCAULONA1000:CCNA_00047-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:CCNA_00047
OrganismiCaulobacter crescentus (strain NA1000 / CB15N)
Taxonomic identifieri565050 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
ProteomesiUP000001364: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226tRNA (guanine-N(7)-)-methyltransferasePRO_1000149650Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi190650.CC_0049.

Structurei

3D structure databases

ProteinModelPortaliB8GX04.
SMRiB8GX04. Positions 56-211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 1446Interaction with RNAUniRule annotation
Regioni206 – 2094Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiYLTFIKK.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8GX04-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTPQQPHGP LRSFGRLKSR PVKPRQQALL DTLLPEIAVP TGPFQPLDLM
60 70 80 90 100
PEAKAVWLEI GFGGGEHMAS QAGRNPETLV IGAEPFVNGV ASAVRHVEEQ
110 120 130 140 150
ALKNVRIHEG DARDVVDWLP DACLDRVFIM FPDPWHKARH NKRRLIQPEF
160 170 180 190 200
VAKLARVMKP GAALRFATDW ADYAEWTTER VLADPSFRFA DEAADRNAIP
210 220
ADHVTTRYEE KKLGDCAPVF LDFTRG
Length:226
Mass (Da):25,322
Last modified:March 3, 2009 - v1
Checksum:i6575F723126A1032
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001340 Genomic DNA. Translation: ACL93514.1.
RefSeqiWP_012639863.1. NC_011916.1.
YP_002515422.1. NC_011916.1.

Genome annotation databases

EnsemblBacteriaiACL93514; ACL93514; CCNA_00047.
GeneIDi7332137.
KEGGiccs:CCNA_00047.
PATRICi21304659. VBICauCre52860_0047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001340 Genomic DNA. Translation: ACL93514.1 .
RefSeqi WP_012639863.1. NC_011916.1.
YP_002515422.1. NC_011916.1.

3D structure databases

ProteinModelPortali B8GX04.
SMRi B8GX04. Positions 56-211.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190650.CC_0049.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL93514 ; ACL93514 ; CCNA_00047 .
GeneIDi 7332137.
KEGGi ccs:CCNA_00047.
PATRICi 21304659. VBICauCre52860_0047.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073969.
KOi K03439.
OMAi YLTFIKK.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci CAULONA1000:CCNA_00047-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genetic basis of laboratory adaptation in Caulobacter crescentus."
    Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S.
    J. Bacteriol. 192:3678-3688(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NA1000 / CB15N.

Entry informationi

Entry nameiTRMB_CAUCN
AccessioniPrimary (citable) accession number: B8GX04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3