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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei59S-adenosyl-L-methionineUniRule annotation1
Binding sitei84S-adenosyl-L-methionineUniRule annotation1
Binding sitei111S-adenosyl-L-methionineUniRule annotation1
Active sitei133By similarity1
Binding sitei133S-adenosyl-L-methionineUniRule annotation1
Binding sitei137SubstrateUniRule annotation1
Binding sitei169SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:CCNA_00047
OrganismiCaulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)
Taxonomic identifieri565050 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001364 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001496501 – 226tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST226

Structurei

3D structure databases

ProteinModelPortaliB8GX04
SMRiB8GX04
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 144Interaction with RNAUniRule annotation6
Regioni206 – 209Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073969
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

B8GX04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPQQPHGP LRSFGRLKSR PVKPRQQALL DTLLPEIAVP TGPFQPLDLM
60 70 80 90 100
PEAKAVWLEI GFGGGEHMAS QAGRNPETLV IGAEPFVNGV ASAVRHVEEQ
110 120 130 140 150
ALKNVRIHEG DARDVVDWLP DACLDRVFIM FPDPWHKARH NKRRLIQPEF
160 170 180 190 200
VAKLARVMKP GAALRFATDW ADYAEWTTER VLADPSFRFA DEAADRNAIP
210 220
ADHVTTRYEE KKLGDCAPVF LDFTRG
Length:226
Mass (Da):25,322
Last modified:March 3, 2009 - v1
Checksum:i6575F723126A1032
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001340 Genomic DNA Translation: ACL93514.1
RefSeqiWP_012639863.1, NC_011916.1
YP_002515422.1, NC_011916.1

Genome annotation databases

EnsemblBacteriaiACL93514; ACL93514; CCNA_00047
GeneIDi7332137
KEGGiccs:CCNA_00047
PATRICifig|565050.3.peg.47

Similar proteinsi

Entry informationi

Entry nameiTRMB_CAUVN
AccessioniPrimary (citable) accession number: B8GX04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 25, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health