Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B8GX04 (TRMB_CAUCN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:CCNA_00047
OrganismCaulobacter crescentus (strain NA1000 / CB15N) [Complete proteome] [HAMAP]
Taxonomic identifier565050 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000149650

Regions

Region139 – 1446Interaction with RNA Potential
Region206 – 2094Substrate binding By similarity

Sites

Active site1331 By similarity
Binding site591S-adenosyl-L-methionine By similarity
Binding site841S-adenosyl-L-methionine By similarity
Binding site1111S-adenosyl-L-methionine By similarity
Binding site1331S-adenosyl-L-methionine By similarity
Binding site1371Substrate By similarity
Binding site1691Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B8GX04 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 6575F723126A1032

FASTA22625,322
        10         20         30         40         50         60 
MTTPQQPHGP LRSFGRLKSR PVKPRQQALL DTLLPEIAVP TGPFQPLDLM PEAKAVWLEI 

        70         80         90        100        110        120 
GFGGGEHMAS QAGRNPETLV IGAEPFVNGV ASAVRHVEEQ ALKNVRIHEG DARDVVDWLP 

       130        140        150        160        170        180 
DACLDRVFIM FPDPWHKARH NKRRLIQPEF VAKLARVMKP GAALRFATDW ADYAEWTTER 

       190        200        210        220 
VLADPSFRFA DEAADRNAIP ADHVTTRYEE KKLGDCAPVF LDFTRG 

« Hide

References

[1]"The genetic basis of laboratory adaptation in Caulobacter crescentus."
Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S.
J. Bacteriol. 192:3678-3688(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NA1000 / CB15N.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001340 Genomic DNA. Translation: ACL93514.1.
RefSeqYP_002515422.1. NC_011916.1.

3D structure databases

ProteinModelPortalB8GX04.
SMRB8GX04. Positions 56-211.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190650.CC_0049.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL93514; ACL93514; CCNA_00047.
GeneID7332137.
KEGGccs:CCNA_00047.
PATRIC21304659. VBICauCre52860_0047.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMAYLTFIKK.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycCAULONA1000:CCNA_00047-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CAUCN
AccessionPrimary (citable) accession number: B8GX04
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways