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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Caulobacter crescentus (strain NA1000 / CB15N)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei59S-adenosyl-L-methionineUniRule annotation1
Binding sitei84S-adenosyl-L-methionineUniRule annotation1
Binding sitei111S-adenosyl-L-methionineUniRule annotation1
Active sitei133By similarity1
Binding sitei133S-adenosyl-L-methionineUniRule annotation1
Binding sitei137SubstrateUniRule annotation1
Binding sitei169SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCAULONA1000:CCNA_00047-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:CCNA_00047
OrganismiCaulobacter crescentus (strain NA1000 / CB15N)
Taxonomic identifieri565050 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001364 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001496501 – 226tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST226

Structurei

3D structure databases

ProteinModelPortaliB8GX04.
SMRiB8GX04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 144Interaction with RNAUniRule annotation6
Regioni206 – 209Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073969.
KOiK03439.
OMAiWPKKKHW.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8GX04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPQQPHGP LRSFGRLKSR PVKPRQQALL DTLLPEIAVP TGPFQPLDLM
60 70 80 90 100
PEAKAVWLEI GFGGGEHMAS QAGRNPETLV IGAEPFVNGV ASAVRHVEEQ
110 120 130 140 150
ALKNVRIHEG DARDVVDWLP DACLDRVFIM FPDPWHKARH NKRRLIQPEF
160 170 180 190 200
VAKLARVMKP GAALRFATDW ADYAEWTTER VLADPSFRFA DEAADRNAIP
210 220
ADHVTTRYEE KKLGDCAPVF LDFTRG
Length:226
Mass (Da):25,322
Last modified:March 3, 2009 - v1
Checksum:i6575F723126A1032
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001340 Genomic DNA. Translation: ACL93514.1.
RefSeqiWP_012639863.1. NC_011916.1.
YP_002515422.1. NC_011916.1.

Genome annotation databases

EnsemblBacteriaiACL93514; ACL93514; CCNA_00047.
GeneIDi7332137.
KEGGiccs:CCNA_00047.
PATRICi21304659. VBICauCre52860_0047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001340 Genomic DNA. Translation: ACL93514.1.
RefSeqiWP_012639863.1. NC_011916.1.
YP_002515422.1. NC_011916.1.

3D structure databases

ProteinModelPortaliB8GX04.
SMRiB8GX04.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL93514; ACL93514; CCNA_00047.
GeneIDi7332137.
KEGGiccs:CCNA_00047.
PATRICi21304659. VBICauCre52860_0047.

Phylogenomic databases

HOGENOMiHOG000073969.
KOiK03439.
OMAiWPKKKHW.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciCAULONA1000:CCNA_00047-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_CAUCN
AccessioniPrimary (citable) accession number: B8GX04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.