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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:CCNA_00013
OrganismiCaulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)
Taxonomic identifieri565050 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001364 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001325271 – 940Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST940

Proteomic databases

PRIDEiB8GWX0

Structurei

3D structure databases

ProteinModelPortaliB8GWX0
SMRiB8GWX0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini496 – 618HDPROSITE-ProRule annotationAdd BLAST123
Domaini737 – 821ACT 1UniRule annotationAdd BLAST85
Domaini848 – 929ACT 2UniRule annotationAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 379UridylyltransferaseAdd BLAST379
Regioni380 – 736Uridylyl-removingAdd BLAST357

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000261779
KOiK00990
OMAiARICGYF
OrthoDBiPOG091H04HO

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 2 hits
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

B8GWX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRRLRPTRL EHVVDGHALR ARLSAAALDS IGNEAEQRAR AIDILKQALF
60 70 80 90 100
RGRMIAKERL ENGASGVETS RLLSGVTDEV ITALYDFTTV HVFRARNPTE
110 120 130 140 150
GERLCLLAVG GYGRGTLAPF SDIDLLFLRP YKQTPHAESV IEYMLYALWD
160 170 180 190 200
LGFKVGHASR TIEECVRLSK EDFTIRTSIL EARRLTGDER LAEDLKKRFR
210 220 230 240 250
DEVMKATGAQ FVAAKLKERD DRQARAGASR YMVEPNVKEG KGGLRDLHTL
260 270 280 290 300
MWIAEYLHPV DRPEDVFKME VFSIRETKAF IRAFDFLHAV RAHLHFTTGR
310 320 330 340 350
PEERLTFDLQ PEIARRMGYG DRGDAPAVER FMRRYFLIAK EVGTLTRAFS
360 370 380 390 400
AKLEAEHFKN EPKGISRFLP GARPKRKALD VEGFYEDGGR LNIEGQEIFE
410 420 430 440 450
ADPVNLIRLF KIADERDLDL HPDAFTAVTR ALPLITSRVR RDPDACRAFL
460 470 480 490 500
DLLARGKRSY RTLTLMNDAG VLGRFIPEFG RVVAQMQFNM YHSYTVDEHT
510 520 530 540 550
LRAVGVIGDI AAGRLVDDHP LAVSIMPLIE DREALFLAML LHDTGKGGVG
560 570 580 590 600
GQEKAGARSA RSACERLGVE RSKVELVAWL VENHLVMSDF AQKRDVSDPG
610 620 630 640 650
TVAAFARIVE NPERLRLLLV ITVADIRAVG PGVWNGWKGQ LLRELYNATE
660 670 680 690 700
AVFRGGRGSD AAANVQRHQE STAEAARAAL LETDPAAKGW VAAMENAYFS
710 720 730 740 750
AFSQDDLFHH AELARRAAIQ GGAAAEGQVR PGSNAAEVVI AAKDRRGLFA
760 770 780 790 800
DLALAISSLG GNVVGARVFT SRQGQALDVF YVQDVTGAPF GCENPRALRR
810 820 830 840 850
LADALEAAGK GDALAVEPRR GSEQTRAAAF AIAPSVTIDN DASNDATVVE
860 870 880 890 900
ASGRDRPGLL HALAKTLADS ALSIQSAHID GYGERAVDAF YVQTTEGGKV
910 920 930 940
TDTRKLNALK ADLLAALEQN EASAPAARPG LRRARASVAR
Length:940
Mass (Da):103,396
Last modified:March 3, 2009 - v1
Checksum:i2F1985420353F8ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001340 Genomic DNA Translation: ACL93480.1
RefSeqiWP_010917903.1, NC_011916.1
YP_002515388.1, NC_011916.1

Genome annotation databases

EnsemblBacteriaiACL93480; ACL93480; CCNA_00013
GeneIDi7332895
KEGGiccs:CCNA_00013
PATRICifig|565050.3.peg.14

Similar proteinsi

Entry informationi

Entry nameiGLND_CAUVN
AccessioniPrimary (citable) accession number: B8GWX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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