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B8GVW2

- DAPF_CAUCN

UniProt

B8GVW2 - DAPF_CAUCN

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Caulobacter crescentus (strain NA1000 / CB15N)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei51 – 511SubstrateUniRule annotation
    Binding sitei71 – 711SubstrateUniRule annotation
    Active sitei80 – 801Proton donor/acceptorUniRule annotation
    Binding sitei166 – 1661SubstrateUniRule annotation
    Sitei168 – 1681Important for catalytic activityUniRule annotation
    Binding sitei200 – 2001SubstrateUniRule annotation
    Sitei218 – 2181Important for catalytic activityUniRule annotation
    Active sitei227 – 2271Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciCAULONA1000:CCNA_03800-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:CCNA_03800
    OrganismiCaulobacter crescentus (strain NA1000 / CB15N)
    Taxonomic identifieri565050 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
    ProteomesiUP000001364: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Diaminopimelate epimerasePRO_1000124406Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi80 ↔ 227

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi190650.CC_3686.

    Structurei

    3D structure databases

    ProteinModelPortaliB8GVW2.
    SMRiB8GVW2. Positions 5-280.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 112Substrate bindingUniRule annotation
    Regioni80 – 823Substrate bindingUniRule annotation
    Regioni218 – 2192Substrate bindingUniRule annotation
    Regioni228 – 2292Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiLIVEPPY.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8GVW2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRTFLKMNG LGNDFVVIQT LTEAFDPTPE QIRAIAKRPG VDGKGGIGCD    50
    QVIAIDPPRA EGASAYVRFW NSDGEVAGAC GNGTRCVAWL LMQSAGKDAV 100
    AFDTVAGRLS GVAAGDKLVT VDMGPPGLDW TQIPLAEEMN TERVELQVGP 150
    IDAPLVHTPV CVSMGNPHVV FFVDAPVTDD FARGTGSLVE HHPLFPEGVN 200
    VGFAHIASRD HIRLKVWERG AGLTQACGTG ACAAQVAAVR RGLTDRKARV 250
    EFDTGSLTIE WRESDGHVIM TGPITMEYAG KLPELVAA 288
    Length:288
    Mass (Da):30,546
    Last modified:March 3, 2009 - v1
    Checksum:i8E47AB6C103BAD88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001340 Genomic DNA. Translation: ACL97265.1.
    RefSeqiWP_010921513.1. NC_011916.1.
    YP_002519173.1. NC_011916.1.

    Genome annotation databases

    EnsemblBacteriaiACL97265; ACL97265; CCNA_03800.
    GeneIDi7331998.
    KEGGiccs:CCNA_03800.
    PATRICi21312087. VBICauCre52860_3704.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001340 Genomic DNA. Translation: ACL97265.1 .
    RefSeqi WP_010921513.1. NC_011916.1.
    YP_002519173.1. NC_011916.1.

    3D structure databases

    ProteinModelPortali B8GVW2.
    SMRi B8GVW2. Positions 5-280.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 190650.CC_3686.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL97265 ; ACL97265 ; CCNA_03800 .
    GeneIDi 7331998.
    KEGGi ccs:CCNA_03800.
    PATRICi 21312087. VBICauCre52860_3704.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi LIVEPPY.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci CAULONA1000:CCNA_03800-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genetic basis of laboratory adaptation in Caulobacter crescentus."
      Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V., Walunas T.L., Crosson S.
      J. Bacteriol. 192:3678-3688(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NA1000 / CB15N.

    Entry informationi

    Entry nameiDAPF_CAUCN
    AccessioniPrimary (citable) accession number: B8GVW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3