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B8GV13 (SYL_THISH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Tgr7_2279
OrganismThioalkalivibrio sp. (strain HL-EbGR7) [Complete proteome] [HAMAP]
Taxonomic identifier396588 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeThioalkalivibrio

Protein attributes

Sequence length817 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 817817Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199231

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B8GV13 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 4D8F3A4160C9299D

FASTA81791,879
        10         20         30         40         50         60 
MQEHYEPDAL ERQAQTWWDE HQTFRAREDA PGEPFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMQG RNVLQPMGWD AFGLPAENAA IKHLVPPAKW TYENIAYMRG QLQRLGFGYD 

       130        140        150        160        170        180 
WDRELATCRP EYYRWEQWLF TRLVKKGLAY KKTAMVNWDP VDQTVLANEQ VIDGKGWRSG 

       190        200        210        220        230        240 
APVERREIPQ WFLRITDYAE ELLKGLDGLE GWPEQVRTMQ RNWIGRSEGV ELEFEVPGEA 

       250        260        270        280        290        300 
PLTVYTTRPD TLMGVSYVGV APEHPLAARA AEGDGKLAAF IEACRHTKVS EADMATLEKK 

       310        320        330        340        350        360 
GMDTGLKAVH PVTGEPVPVW VANFVLMEYG TGAVMAVPAH DQRDWEFARQ YGLPVRQVIL 

       370        380        390        400        410        420 
PAEEGVAVDL DQAAFTDKGV LIESAQFNGL SSAQAFDAIA DFLEKQHKGR RRVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGC PIPIINCPDC GPVPVPEEQL PVVLPEDVAF DGVGSPIKRM PEFIDTTCPE 

       490        500        510        520        530        540 
CGGKAERETD TFDTFFESSW YYARYTCKDA DGAMLDERAR HWLPVDQYIG GIEHAVLHLL 

       550        560        570        580        590        600 
YARFFHKLMR DEGLVDGDEP FTRLLTQGMV LKDGAKMSKS KGNTVDPEAL IERYGADTVR 

       610        620        630        640        650        660 
LFMMFAAPPE LSLEWSDSGV EGAYRFLKRL WKQVHDHVQG GAAPALDKAA LNADQQALRR 

       670        680        690        700        710        720 
KLHQTLAKVS DDIGRRTTFN TAIAACMELM NELGRFEDKS GQGRAVMQEA LETTVLMLSP 

       730        740        750        760        770        780 
IVPHIAHALW SELGREGAAV DQPWPVVDES ALESDTVELV VQVNGKLRAQ IQVPAAAARA 

       790        800        810 
AIEEAALADE NVQRHIEGKT VVKMVVVPGR LVNVVVK 

« Hide

References

[1]"Complete genome sequence of 'Thioalkalivibrio sulfidophilus' HL-EbGr7."
Muyzer G., Sorokin D.Y., Mavromatis K., Lapidus A., Clum A., Ivanova N., Pati A., d'Haeseleer P., Woyke T., Kyrpides N.C.
Stand. Genomic Sci. 4:23-35(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HL-EbGR7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001339 Genomic DNA. Translation: ACL73359.1.
RefSeqYP_002514346.1. NC_011901.1.

3D structure databases

ProteinModelPortalB8GV13.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING396588.Tgr7_2279.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL73359; ACL73359; Tgr7_2279.
GeneID7317528.
KEGGtgr:Tgr7_2279.
PATRIC23964084. VBIThiSp19295_2273.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycTSUL396588:GH5B-2313-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_THISH
AccessionPrimary (citable) accession number: B8GV13
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries