Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Siroheme synthase

Gene

cysG

Organism
Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

Catalytic activityi

2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei227S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei250Proton acceptorUniRule annotation1
Active sitei272Proton donorUniRule annotation1
Binding sitei308S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei385S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei414S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADUniRule annotation2
Nucleotide bindingi43 – 44NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Methyltransferase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processCobalamin biosynthesis, Porphyrin biosynthesis
LigandNAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTSUL396588:G1GUK-2201-MONOMER
UniPathwayiUPA00148; UER00211
UPA00148; UER00222
UPA00262; UER00211
UPA00262; UER00222
UPA00262; UER00376

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseUniRule annotation
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferaseUniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylaseUniRule annotation
Alternative name(s):
SUMTUniRule annotation
Uroporphyrinogen III methylaseUniRule annotation
Short name:
UROMUniRule annotation
Precorrin-2 dehydrogenaseUniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelataseUniRule annotation (EC:4.99.1.4UniRule annotation)
Gene namesi
Name:cysGUniRule annotation
Ordered Locus Names:Tgr7_2173
OrganismiThioalkalivibrio sulfidiphilus (strain HL-EbGR7)
Taxonomic identifieri396588 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeThioalkalivibrio
Proteomesi
  • UP000002383 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001869601 – 489Siroheme synthaseAdd BLAST489

Interactioni

Protein-protein interaction databases

STRINGi396588.Tgr7_2173

Structurei

3D structure databases

SMRiB8GUD3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd BLAST203
Regioni218 – 489Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd BLAST272
Regioni303 – 305S-adenosyl-L-methionine bindingUniRule annotation3
Regioni333 – 334S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CB8 Bacteria
COG0007 LUCA
COG1648 LUCA
KOiK02302
OMAiFCVRADD
OrthoDBiPOG091H0526

Family and domain databases

CDDicd11642 SUMT, 1 hit
Gene3Di1.10.8.210, 1 hit
3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01646 Siroheme_synth, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR006366 CobA/CysG_C
IPR036291 NAD(P)-bd_dom_sf
IPR037115 Sirohaem_synt_dimer_dom_sf
IPR012409 Sirohaem_synth
IPR028281 Sirohaem_synthase_central
IPR019478 Sirohaem_synthase_dimer_dom
IPR006367 Sirohaem_synthase_N
IPR003043 Uropor_MeTrfase_CS
PfamiView protein in Pfam
PF10414 CysG_dimeriser, 1 hit
PF14824 Sirohm_synth_M, 1 hit
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036426 Sirohaem_synth, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR01469 cobA_cysG_Cterm, 1 hit
TIGR01470 cysG_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS00840 SUMT_2, 1 hit

Sequencei

Sequence statusi: Complete.

B8GUD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFFPVFMRL KDRACLVVGG GPVALRKVSL LRKAHARVTV VSPALCPELA
60 70 80 90 100
ALKARDAIEH LPRGFEDADV DARVLVIAAT DDEDLNRHVS ELCTERCIPV
110 120 130 140 150
NVVDQPELCG FITPSMIDRS PIQVAVSTGG SSPVLARLLR SRIESFIPAA
160 170 180 190 200
YGRLAALVEG YRLKVKARIP DTELRRRFWE RLLEGPVTEL FMSGREDAAR
210 220 230 240 250
ETLEKALETG LDTRDAGGEV FLVGAGPGDP DLLTFRALRL MQLADVVVYD
260 270 280 290 300
NLVSPAIIEL VRRDAEMIYA GKKRNLHTLP QEEINQLLVR LAKEGKRVLR
310 320 330 340 350
LKGGDPFIFG RGGEEIDTLM QEGIPFQVVP GITAAAGCAS FSGIPLTHRD
360 370 380 390 400
YAQAVVFATG HLRDGSIDLN WKMLAQPRQT VVFYMGLLGL PIICRELMAH
410 420 430 440 450
GVSPDMPMAL VEQGTTQNQR VIVGTLASMP DLVKDYDVQP PTLIIVGEVV
460 470 480
KLHERLKWFQ PGTDHTVKSL FSSQGQVVEA RSSTEGAEA
Length:489
Mass (Da):53,653
Last modified:March 3, 2009 - v1
Checksum:i93839C50809409A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001339 Genomic DNA Translation: ACL73253.1
RefSeqiWP_012638731.1, NC_011901.1

Genome annotation databases

EnsemblBacteriaiACL73253; ACL73253; Tgr7_2173
KEGGitgr:Tgr7_2173

Similar proteinsi

Entry informationi

Entry nameiCYSG_THISH
AccessioniPrimary (citable) accession number: B8GUD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: March 28, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health