B8GTR0 (B8GTR0_THISH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685
Ordered Locus Names:	Tgr7_2074

Organism

Thioalkalivibrio sp. (strain HL-EbGR7) [Complete proteome] [HAMAP]

Taxonomic identifier

396588 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Thioalkalivibrio

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	407	1	Nucleophile By similarity HAMAP MF_00685
	Active site	460	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B8GTR0 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 9204FCA58005FE26	FASTA	725	83,807
	10 20 30 40 50 60 MKVQEKSMIP SEIRRILDAR HHDPFSVLGY HEIGGEFLVR SFLPQAAEAW VVEAGDAPME 70 80 90 100 110 120 RLEGTDLFEW RGDALPRPYR IRWSDGHGHE HVAWDPYGFE PLLSDFDIHL FNEGRHWHAW 130 140 150 160 170 180 RLMGAHVQSV GEVDGVRFTV WAPGAERVSV VGDFNRWDGR CHPMRVRGGS GIWELFIPGL 190 200 210 220 230 240 EPGCLYKYEI RNRDSGEIMV KTDPYGQNFE LRPKTAGVVP APADYDWQDG EWLARRHGDA 250 260 270 280 290 300 WLHRPMSIYE VHLGSWRRGP EGEFLGYREL AHALVDYVKQ LGFTHIELLP VTEHPFDASW 310 320 330 340 350 360 GYQTTGYYAP TSRFGTPEDF RYFVDHCHAN DIGVILDWAP GHFPKDRHAL ARFDGTALYE 370 380 390 400 410 420 HEDPRKGEHR DWGTLIFNYG RNEVRNFLVS SALYWVEEFH IDGLRVDAVA SMLYLDYSRE 430 440 450 460 470 480 PGDWEPNRYG GNENLEAIDF IRELNNVVQG QHPGALIIAE ESTSWPQVTR PTWLGGLGFS 490 500 510 520 530 540 MKWNMGWMHD TLEYFKKDPI HRHYHHDQLT FGLLYAFTEN FVLPFSHDEV VHGKRSLLYR 550 560 570 580 590 600 MPGDEWQRFA NLRLLYTYMW AYPGKKLLFM GSEFGQGDEW DAANQLDWYV LDYPLHQGMQ 610 620 630 640 650 660 HLVGDLNRLY RDHPALHQHD FDWQGFEWID CHDAAQSVLS FLRKDDDELM VVALSFTPVP 670 680 690 700 710 720 REGYRLGVPR PGRYQVVLNS DSSHYGGSNL GQPAAQSEDI PWMGHPHSIV LTLPPLGGLI LRHSG

References

[1]

"Complete genome sequence of 'Thioalkalivibrio sulfidophilus' HL-EbGr7." Muyzer G., Sorokin D.Y., Mavromatis K., Lapidus A., Clum A., Ivanova N., Pati A., d'Haeseleer P., Woyke T., Kyrpides N.C. Stand. Genomic Sci. 4:23-35(2011) [PubMed: 21475584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HL-EbGR7 EMBL ACL73154.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001339 Genomic DNA. Translation: ACL73154.1.
RefSeq	YP_002514141.1. NC_011901.1.
3D structure databases
ProteinModelPortal	B8GTR0.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GTR0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7316464.
GenomeReviews	Gene locus Tgr7_2074 in contig CP001339_GR.
KEGG	tgr:Tgr7_2074.
PATRIC	23963664. VBIThiSp19295_2066.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	DGTCLYE.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8GTR0_THISH

Accession

Primary (citable) accession number: B8GTR0

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)