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B8GTH4

- BIOB_THISH

UniProt

B8GTH4 - BIOB_THISH

Protein

Biotin synthase

Gene

bioB

Organism
Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi104 – 1041Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi135 – 1351Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi195 – 1951Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi267 – 2671Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciTSUL396588:GH5B-131-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Tgr7_0131
    OrganismiThioalkalivibrio sulfidiphilus (strain HL-EbGR7)
    Taxonomic identifieri396588 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeThioalkalivibrio
    ProteomesiUP000002383: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 340340Biotin synthasePRO_0000381691Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi396588.Tgr7_0131.

    Structurei

    3D structure databases

    ProteinModelPortaliB8GTH4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B8GTH4-1 [UniParc]FASTAAdd to Basket

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    MSPASPMSEI RHDWQVDEIL ELMGRPFNDL LFAAHTVHRA HFDPNAVQVS    50
    TLLSIKTGAC PEDCGYCPQS AHHEVELERE RLLPLEEVLE AARNARSHGA 100
    SRFCMGAAWR NPTDRNLERV IEMVEGVKQL GLETCMTLGM LTDAQARRLK 150
    DAGLDYYNHN LDTSPEFYGQ VITTRTYQDR LETLAHVREA GINVCSGGIL 200
    GMGESRRDRA RLLQQLANLP RHPESVPINN LVQVEGTPLA GTEALDPFEF 250
    VRTIATARIL MPASYVRLSA GRTEMHDELQ ALCFFAGANS IFYGDKLLTT 300
    PNPGENHDRA LFERLGIHPL EHSDEGASCE TCGGVDIHAA 340
    Length:340
    Mass (Da):37,709
    Last modified:March 3, 2009 - v1
    Checksum:i032597EDCA6DFF99
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001339 Genomic DNA. Translation: ACL71234.1.
    RefSeqiYP_002512221.1. NC_011901.1.

    Genome annotation databases

    EnsemblBacteriaiACL71234; ACL71234; Tgr7_0131.
    GeneIDi7316137.
    KEGGitgr:Tgr7_0131.
    PATRICi23959752. VBIThiSp19295_0136.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001339 Genomic DNA. Translation: ACL71234.1 .
    RefSeqi YP_002512221.1. NC_011901.1.

    3D structure databases

    ProteinModelPortali B8GTH4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 396588.Tgr7_0131.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL71234 ; ACL71234 ; Tgr7_0131 .
    GeneIDi 7316137.
    KEGGi tgr:Tgr7_0131.
    PATRICi 23959752. VBIThiSp19295_0136.

    Phylogenomic databases

    eggNOGi COG0502.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci TSUL396588:GH5B-131-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HL-EbGR7.

    Entry informationi

    Entry nameiBIOB_THISH
    AccessioniPrimary (citable) accession number: B8GTH4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3