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B8GRB6

- GLMU_THISH

UniProt

B8GRB6 - GLMU_THISH

Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
    UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei24 – 241UDP-GlcNAcUniRule annotation
    Binding sitei75 – 751UDP-GlcNAcUniRule annotation
    Metal bindingi104 – 1041MagnesiumUniRule annotation
    Binding sitei140 – 1401UDP-GlcNAc; via amide nitrogenUniRule annotation
    Binding sitei155 – 1551UDP-GlcNAcUniRule annotation
    Binding sitei170 – 1701UDP-GlcNAcUniRule annotation
    Metal bindingi228 – 2281MagnesiumUniRule annotation
    Binding sitei228 – 2281UDP-GlcNAcUniRule annotation
    Binding sitei334 – 3341Acetyl-CoA; amide nitrogenUniRule annotation
    Binding sitei352 – 3521Acetyl-CoAUniRule annotation
    Active sitei364 – 3641Proton acceptorUniRule annotation
    Binding sitei367 – 3671Acetyl-CoAUniRule annotation
    Binding sitei378 – 3781Acetyl-CoAUniRule annotation
    Binding sitei406 – 4061Acetyl-CoAUniRule annotation
    Binding sitei424 – 4241Acetyl-CoA; via amide nitrogenUniRule annotation
    Binding sitei441 – 4411Acetyl-CoAUniRule annotation

    GO - Molecular functioni

    1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell morphogenesis Source: UniProtKB-HAMAP
    2. lipid A biosynthetic process Source: UniProtKB-UniPathway
    3. lipopolysaccharide biosynthetic process Source: InterPro
    4. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    5. regulation of cell shape Source: UniProtKB-KW
    6. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciTSUL396588:GH5B-3355-MONOMER.
    UniPathwayiUPA00113; UER00532.
    UPA00113; UER00533.
    UPA00973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional protein GlmUUniRule annotation
    Including the following 2 domains:
    UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
    Alternative name(s):
    N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
    Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
    Gene namesi
    Name:glmUUniRule annotation
    Ordered Locus Names:Tgr7_3303
    OrganismiThioalkalivibrio sulfidiphilus (strain HL-EbGR7)
    Taxonomic identifieri396588 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeThioalkalivibrio
    ProteomesiUP000002383: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 459459Bifunctional protein GlmUPRO_1000186508Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi396588.Tgr7_3303.

    Structurei

    3D structure databases

    ProteinModelPortaliB8GRB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 230230PyrophosphorylaseUniRule annotationAdd
    BLAST
    Regioni10 – 134UDP-GlcNAc bindingUniRule annotation
    Regioni80 – 812UDP-GlcNAc bindingUniRule annotation
    Regioni102 – 1043UDP-GlcNAc bindingUniRule annotation
    Regioni231 – 25121LinkerUniRule annotationAdd
    BLAST
    Regioni252 – 459208N-acetyltransferaseUniRule annotationAdd
    BLAST
    Regioni387 – 3882Acetyl-CoA bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
    In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1207.
    KOiK04042.
    OMAiDCVTNQD.
    OrthoDBiEOG6Z6FQZ.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    HAMAPiMF_01631. GlmU.
    InterProiIPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view]
    PfamiPF00132. Hexapep. 4 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsiTIGR01173. glmU. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B8GRB6-1 [UniParc]FASTAAdd to Basket

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    MTTPTSILIL AAGQGTRMRS SLPKVLQPLA GRPLLSHVLD TARSLDPHRL    50
    VVVYGHGGDR VREAFPEDDI RWVHQAEQRG TGHAVQVALP ETDADDRVLI 100
    LYGDVPLVRG ETVRRLLAQL DEGGDLVILT AALKSPTGYG RILRDDSGRV 150
    QRIVEEKDAS DSERRVNEVN TGIMAARASD FTRWLSKVTN DNAQGEFYLT 200
    DCVALANTEG RRVEAVVGHE AIELMGVNDK RQLAEQERAY QRREAERLMV 250
    QGVTVIDPAR LDVRGEVEAG RDVSLDINVI LQGSVKLGEG AKVGAGCVII 300
    DSEIGPGAHI LPHTVIEGAV IGAGASVGPF ARIRPGTHTD SNAKIGNFVE 350
    VKNARVGEGS KINHLSYVGD SELGRDVNIG AGTITCNYDG ANKHKTIIGD 400
    RAFIGSNTAL VAPLTVGEGA TIGAGTTLNK DAPPGELTVA RAKAITIPGW 450
    KRPVKKPKE 459
    Length:459
    Mass (Da):49,073
    Last modified:March 3, 2009 - v1
    Checksum:i6D7E892C8B1ED632
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001339 Genomic DNA. Translation: ACL74370.1.
    RefSeqiYP_002515357.1. NC_011901.1.

    Genome annotation databases

    EnsemblBacteriaiACL74370; ACL74370; Tgr7_3303.
    GeneIDi7315531.
    KEGGitgr:Tgr7_3303.
    PATRICi23966096. VBIThiSp19295_3262.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001339 Genomic DNA. Translation: ACL74370.1 .
    RefSeqi YP_002515357.1. NC_011901.1.

    3D structure databases

    ProteinModelPortali B8GRB6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 396588.Tgr7_3303.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL74370 ; ACL74370 ; Tgr7_3303 .
    GeneIDi 7315531.
    KEGGi tgr:Tgr7_3303.
    PATRICi 23966096. VBIThiSp19295_3262.

    Phylogenomic databases

    eggNOGi COG1207.
    KOi K04042.
    OMAi DCVTNQD.
    OrthoDBi EOG6Z6FQZ.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00532 .
    UPA00113 ; UER00533 .
    UPA00973 .
    BioCyci TSUL396588:GH5B-3355-MONOMER.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    HAMAPi MF_01631. GlmU.
    InterProi IPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view ]
    Pfami PF00132. Hexapep. 4 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsi TIGR01173. glmU. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HL-EbGR7.

    Entry informationi

    Entry nameiGLMU_THISH
    AccessioniPrimary (citable) accession number: B8GRB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3