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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei560Aminoacyl-adenylateUniRule annotation1
Binding sitei604ATPUniRule annotation1
Metal bindingi900ZincUniRule annotation1
Metal bindingi903ZincUniRule annotation1
Metal bindingi920ZincUniRule annotation1
Metal bindingi923ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTSUL396588:G1GUK-3264-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Tgr7_3216
OrganismiThioalkalivibrio sulfidiphilus (strain HL-EbGR7)
Taxonomic identifieri396588 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeThioalkalivibrio
Proteomesi
  • UP000002383 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001892121 – 937Isoleucine--tRNA ligaseAdd BLAST937

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi396588.Tgr7_3216

Structurei

3D structure databases

ProteinModelPortaliB8GQR1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi601 – 605"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B8GQR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKDTLNL PQTEFPMRGN LAQREPEMLA HWEAAKRYRK LREVCAGRPR
60 70 80 90 100
FVLADGPPYA NGNIHIGHAV NKILKDIIVK SRTLAGFDAA YIPGWDCHGL
110 120 130 140 150
PIELQVEKKI GKVGHEVDAA AFRKACREYA LEQVDSQRQD FKRLGVLGDW
160 170 180 190 200
EKPYLTMDYQ VEADTVRALG RIIERGHLMK GEKPVHWCVD CGSALAEAEV
210 220 230 240 250
EYEDKTSQAI DVRFRVEDEL DLLRRCALDG DGEGPISVVI WTTTPWTLPA
260 270 280 290 300
NQAVCLHPEL SYALVRVGDE RLLLAEELVE PAMKRYGFSD ARIIGRCQGA
310 320 330 340 350
ALEGALLHHP FLDRQVPVIL GEHVTLEAGT GCVHTAPGHG QDDYIVGLRY
360 370 380 390 400
GLAVDNPVGP DGKFLPGTEF FAGESVHQAN GHVIEVLRER GALVLAEKLR
410 420 430 440 450
HSYPHCWRHK TPIIFRATPQ WFISMEQAEL REQALKAIQQ VQWVPQWGQA
460 470 480 490 500
RIEGMVSGRP DWCISRQRNW GVPIPLFVHL ETGRLHPDTP ALIEAVAQRI
510 520 530 540 550
EAQGIEAWFS LDPQELLGKD AAAYQKVSDT LDVWFDSGVT HATVTDKRAE
560 570 580 590 600
LGLPADLYLE GSDQHRGWFQ SSLLTSVAMR GTAPYKAVLT HGFTVDAQGQ
610 620 630 640 650
KMSKSKGNVV APQKVVDTLG ADILRLWVAA TDYSAEMAVS DEILKRTADA
660 670 680 690 700
YRRMRNTARF LLANLTGFDP ARDMLAPGQM LPLDRWAVDQ ALKVQQKVTG
710 720 730 740 750
AYEQYQFHQI YQRVHNFCSV ELGSFYLDVI KDRQYTTKAD SIARRSAQTA
760 770 780 790 800
MYHIIEAMVR WLAPILSFTA EEIWQEIPGE RNESVLFNTW YQGLFALDAS
810 820 830 840 850
EPMNEAFWAT LLDVRQAVNR EMEKLRAAKA SSLDAEVDLY CEPALAETLE
860 870 880 890 900
GLGEELRFVL ITSYARVHGA ASRPDDAVET PLEDGTSLWT RVTRSEHAKC
910 920 930
PRCWHHREDI GASSDHPELC GRCVENVAGD GEQRRFA
Length:937
Mass (Da):105,192
Last modified:March 3, 2009 - v1
Checksum:iF991D8DBBACF56B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001339 Genomic DNA Translation: ACL74285.1
RefSeqiWP_012639747.1, NC_011901.1

Genome annotation databases

EnsemblBacteriaiACL74285; ACL74285; Tgr7_3216
KEGGitgr:Tgr7_3216

Similar proteinsi

Entry informationi

Entry nameiSYI_THISH
AccessioniPrimary (citable) accession number: B8GQR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: March 28, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health