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B8GQR1

- SYI_THISH

UniProt

B8GQR1 - SYI_THISH

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Tgr7_3216
Organism
Thioalkalivibrio sp. (strain HL-EbGR7)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei560 – 5601Aminoacyl-adenylate By similarity
Binding sitei604 – 6041ATP By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTSUL396588:GH5B-3266-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Tgr7_3216
OrganismiThioalkalivibrio sp. (strain HL-EbGR7)
Taxonomic identifieri396588 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeThioalkalivibrio
ProteomesiUP000002383: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189212Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi396588.Tgr7_3216.

Structurei

3D structure databases

ProteinModelPortaliB8GQR1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi601 – 6055"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8GQR1-1 [UniParc]FASTAAdd to Basket

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MTDYKDTLNL PQTEFPMRGN LAQREPEMLA HWEAAKRYRK LREVCAGRPR    50
FVLADGPPYA NGNIHIGHAV NKILKDIIVK SRTLAGFDAA YIPGWDCHGL 100
PIELQVEKKI GKVGHEVDAA AFRKACREYA LEQVDSQRQD FKRLGVLGDW 150
EKPYLTMDYQ VEADTVRALG RIIERGHLMK GEKPVHWCVD CGSALAEAEV 200
EYEDKTSQAI DVRFRVEDEL DLLRRCALDG DGEGPISVVI WTTTPWTLPA 250
NQAVCLHPEL SYALVRVGDE RLLLAEELVE PAMKRYGFSD ARIIGRCQGA 300
ALEGALLHHP FLDRQVPVIL GEHVTLEAGT GCVHTAPGHG QDDYIVGLRY 350
GLAVDNPVGP DGKFLPGTEF FAGESVHQAN GHVIEVLRER GALVLAEKLR 400
HSYPHCWRHK TPIIFRATPQ WFISMEQAEL REQALKAIQQ VQWVPQWGQA 450
RIEGMVSGRP DWCISRQRNW GVPIPLFVHL ETGRLHPDTP ALIEAVAQRI 500
EAQGIEAWFS LDPQELLGKD AAAYQKVSDT LDVWFDSGVT HATVTDKRAE 550
LGLPADLYLE GSDQHRGWFQ SSLLTSVAMR GTAPYKAVLT HGFTVDAQGQ 600
KMSKSKGNVV APQKVVDTLG ADILRLWVAA TDYSAEMAVS DEILKRTADA 650
YRRMRNTARF LLANLTGFDP ARDMLAPGQM LPLDRWAVDQ ALKVQQKVTG 700
AYEQYQFHQI YQRVHNFCSV ELGSFYLDVI KDRQYTTKAD SIARRSAQTA 750
MYHIIEAMVR WLAPILSFTA EEIWQEIPGE RNESVLFNTW YQGLFALDAS 800
EPMNEAFWAT LLDVRQAVNR EMEKLRAAKA SSLDAEVDLY CEPALAETLE 850
GLGEELRFVL ITSYARVHGA ASRPDDAVET PLEDGTSLWT RVTRSEHAKC 900
PRCWHHREDI GASSDHPELC GRCVENVAGD GEQRRFA 937
Length:937
Mass (Da):105,192
Last modified:March 3, 2009 - v1
Checksum:iF991D8DBBACF56B7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001339 Genomic DNA. Translation: ACL74285.1.
RefSeqiYP_002515272.1. NC_011901.1.

Genome annotation databases

EnsemblBacteriaiACL74285; ACL74285; Tgr7_3216.
GeneIDi7315589.
KEGGitgr:Tgr7_3216.
PATRICi23965932. VBIThiSp19295_3182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001339 Genomic DNA. Translation: ACL74285.1 .
RefSeqi YP_002515272.1. NC_011901.1.

3D structure databases

ProteinModelPortali B8GQR1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 396588.Tgr7_3216.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL74285 ; ACL74285 ; Tgr7_3216 .
GeneIDi 7315589.
KEGGi tgr:Tgr7_3216.
PATRICi 23965932. VBIThiSp19295_3182.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TSUL396588:GH5B-3266-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HL-EbGR7.

Entry informationi

Entry nameiSYI_THISH
AccessioniPrimary (citable) accession number: B8GQR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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