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B8GQR1

- SYI_THISH

UniProt

B8GQR1 - SYI_THISH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei560 – 5601Aminoacyl-adenylateUniRule annotation
    Binding sitei604 – 6041ATPUniRule annotation
    Metal bindingi900 – 9001ZincUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi920 – 9201ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTSUL396588:GH5B-3266-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Tgr7_3216
    OrganismiThioalkalivibrio sulfidiphilus (strain HL-EbGR7)
    Taxonomic identifieri396588 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeThioalkalivibrio
    ProteomesiUP000002383: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000189212Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi396588.Tgr7_3216.

    Structurei

    3D structure databases

    ProteinModelPortaliB8GQR1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi601 – 6055"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8GQR1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDYKDTLNL PQTEFPMRGN LAQREPEMLA HWEAAKRYRK LREVCAGRPR    50
    FVLADGPPYA NGNIHIGHAV NKILKDIIVK SRTLAGFDAA YIPGWDCHGL 100
    PIELQVEKKI GKVGHEVDAA AFRKACREYA LEQVDSQRQD FKRLGVLGDW 150
    EKPYLTMDYQ VEADTVRALG RIIERGHLMK GEKPVHWCVD CGSALAEAEV 200
    EYEDKTSQAI DVRFRVEDEL DLLRRCALDG DGEGPISVVI WTTTPWTLPA 250
    NQAVCLHPEL SYALVRVGDE RLLLAEELVE PAMKRYGFSD ARIIGRCQGA 300
    ALEGALLHHP FLDRQVPVIL GEHVTLEAGT GCVHTAPGHG QDDYIVGLRY 350
    GLAVDNPVGP DGKFLPGTEF FAGESVHQAN GHVIEVLRER GALVLAEKLR 400
    HSYPHCWRHK TPIIFRATPQ WFISMEQAEL REQALKAIQQ VQWVPQWGQA 450
    RIEGMVSGRP DWCISRQRNW GVPIPLFVHL ETGRLHPDTP ALIEAVAQRI 500
    EAQGIEAWFS LDPQELLGKD AAAYQKVSDT LDVWFDSGVT HATVTDKRAE 550
    LGLPADLYLE GSDQHRGWFQ SSLLTSVAMR GTAPYKAVLT HGFTVDAQGQ 600
    KMSKSKGNVV APQKVVDTLG ADILRLWVAA TDYSAEMAVS DEILKRTADA 650
    YRRMRNTARF LLANLTGFDP ARDMLAPGQM LPLDRWAVDQ ALKVQQKVTG 700
    AYEQYQFHQI YQRVHNFCSV ELGSFYLDVI KDRQYTTKAD SIARRSAQTA 750
    MYHIIEAMVR WLAPILSFTA EEIWQEIPGE RNESVLFNTW YQGLFALDAS 800
    EPMNEAFWAT LLDVRQAVNR EMEKLRAAKA SSLDAEVDLY CEPALAETLE 850
    GLGEELRFVL ITSYARVHGA ASRPDDAVET PLEDGTSLWT RVTRSEHAKC 900
    PRCWHHREDI GASSDHPELC GRCVENVAGD GEQRRFA 937
    Length:937
    Mass (Da):105,192
    Last modified:March 3, 2009 - v1
    Checksum:iF991D8DBBACF56B7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001339 Genomic DNA. Translation: ACL74285.1.
    RefSeqiYP_002515272.1. NC_011901.1.

    Genome annotation databases

    EnsemblBacteriaiACL74285; ACL74285; Tgr7_3216.
    GeneIDi7315589.
    KEGGitgr:Tgr7_3216.
    PATRICi23965932. VBIThiSp19295_3182.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001339 Genomic DNA. Translation: ACL74285.1 .
    RefSeqi YP_002515272.1. NC_011901.1.

    3D structure databases

    ProteinModelPortali B8GQR1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 396588.Tgr7_3216.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL74285 ; ACL74285 ; Tgr7_3216 .
    GeneIDi 7315589.
    KEGGi tgr:Tgr7_3216.
    PATRICi 23965932. VBIThiSp19295_3182.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TSUL396588:GH5B-3266-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HL-EbGR7.

    Entry informationi

    Entry nameiSYI_THISH
    AccessioniPrimary (citable) accession number: B8GQR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3