B8GPM6 (KATG_THISH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Tgr7_1107

Organism

Thioalkalivibrio sp. (strain HL-EbGR7) [Complete proteome] [HAMAP]

Taxonomic identifier

396588 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Thioalkalivibrio

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Catalase-peroxidase HAMAP MF_01961		PRO_1000189074
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8GPM6 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 1ED29805F0E765FC	FASTA	723	79,965
	10 20 30 40 50 60 MSAQNIQSAG KCPVMHGANT EVGTSNMDWW PKALNLDILH QHDTKTNPMD EEFSYREAVK 70 80 90 100 110 120 TLDFAAVKRD VEALMTDSQA WWPADWGHYG GLMIRMAWHA AGSYRAADGR GGANTGNQRF 130 140 150 160 170 180 APLNSWPDNA NLDKARRLLW PIKKKYGNRL SWADLIVLAG NVAYESMGLK TFGFGFGRED 190 200 210 220 230 240 IWHPEKDTYW GAEKEWLAPS DERYGSVDDP STMENPLAAV QMGLIYVNPE GVNGKPDPLK 250 260 270 280 290 300 TAAQVRETFA RMGMNDEETV ALTAGGHTVG KTHGNGRAEN LGPSPEGADI SEQGLGWMNH 310 320 330 340 350 360 KTRGIGRDSV TSGIEGAWTT HPTKWDNGYF DMLFGHEWEL KKSPAGAWQW EPVDIREEDM 370 380 390 400 410 420 PVDVEDPSIR CKPIMTDADM AMREDPIYRK ISERFHKDPA LLTETFARAW FKLIHRDMGP 430 440 450 460 470 480 KARYIGPEVP AEDLIWQDPV PAGKTGYDVD AVKAKIAASG LSIAEMVATA WDSARTFRQS 490 500 510 520 530 540 DFRGGANGAR IRLAPQKDWE GNEPARLQKV LAVLEPIAAD SGASVADVIV LAGNVGIEQA 550 560 570 580 590 600 AKAAGVNVTV PFAPGRGDAT QEMTDADGFA VLEPIHDGYR NWVKKDYVVS AEELMLDRTQ 610 620 630 640 650 660 LMGLTAAEMT VLVGGMRVLG TNHGGAKHGV FTDKVGTLSQ DFFVNLTDMN NVWKPAGAHY 670 680 690 700 710 720 EIRDRKTDAV KWTATRVDLV FGSNSILRAY AEVYAQDDNQ EKFVRDFVNA WTKVMNADRF DLK

References

[1]

"Complete genome sequence of 'Thioalkalivibrio sulfidophilus' HL-EbGr7." Muyzer G., Sorokin D.Y., Mavromatis K., Lapidus A., Clum A., Ivanova N., Pati A., d'Haeseleer P., Woyke T., Kyrpides N.C. Stand. Genomic Sci. 4:23-35(2011) [PubMed: 21475584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HL-EbGR7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001339 Genomic DNA. Translation: ACL72193.1.
RefSeq	YP_002513180.1. NC_011901.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8GPM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7315816.
GenomeReviews	Gene locus Tgr7_1107 in contig CP001339_GR.
KEGG	tgr:Tgr7_1107.
PATRIC	23961732. VBIThiSp19295_1112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_THISH

Accession

Primary (citable) accession number: B8GPM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program