B8GPC3 (MDH_THISH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Tgr7_2971

Organism

Thioalkalivibrio sp. (strain HL-EbGR7) [Complete proteome] [HAMAP]

Taxonomic identifier

396588 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Thioalkalivibrio › Thioalkalivibrio sulfidophilus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_1000191659
Regions
	Nucleotide binding	8 – 13	6	NAD By similarity
	Nucleotide binding	118 – 120	3	NAD By similarity
Sites
	Active site	175	1	Proton acceptor By similarity
	Binding site	33	1	NAD By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	95	1	NAD By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8GPC3 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 6C0E53D8449029A2	FASTA	307	32,870
	10 20 30 40 50 60 MEKIAIIGAG RVGESTAQFL AKNDTCRELV LLDVREGAAE GAALDIQETA PLFGFDTRLK 70 80 90 100 110 120 GGTDAAILSG AELVVITAGI PRKPGMSRSD VLDTNVAILD KLVDGIMEHA PDAMLLLVSN 130 140 150 160 170 180 PVDVLTYRAW QRTGWPRNRV FGQAGVLDSS RMASFVALET GLSVNDINAM VLGGHGDSMV 190 200 210 220 230 240 PMLRYSTING IPVRHFLSEE AIARIVERTR HGGAEILALK QTSSAYDAPA AAIAAMVDAI 250 260 270 280 290 300 ALDRKRVLPT VALLEGEYGE RDVAMGVPCI LGRNGVESVI ELPLEPSERK EFDQSLAGVR DDINRLK

References

[1]

"Complete genome sequence of 'Thioalkalivibrio sulfidophilus' HL-EbGr7." Muyzer G., Sorokin D.Y., Mavromatis K., Lapidus A., Clum A., Ivanova N., Pati A., d'Haeseleer P., Woyke T., Kyrpides N.C. Stand. Genomic Sci. 4:23-35(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HL-EbGR7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001339 Genomic DNA. Translation: ACL74043.1.
RefSeq	YP_002515030.1. NC_011901.1.
3D structure databases
ProteinModelPortal	B8GPC3.
ModBase	Search...
Protein-protein interaction databases
STRING	396588.Tgr7_2971.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL74043; ACL74043; Tgr7_2971.
GeneID	7318268.
KEGG	tgr:Tgr7_2971.
PATRIC	23965436. VBIThiSp19295_2937.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
KO	K00024.
OMA	INDYEES.
ProtClustDB	CLSK2464565.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_THISH

Accession

Primary (citable) accession number: B8GPC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program