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B8GP06

- PNP_THISH

UniProt

B8GP06 - PNP_THISH

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi486 – 4861MagnesiumUniRule annotation
    Metal bindingi492 – 4921MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciTSUL396588:GH5B-1021-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:Tgr7_1007
    OrganismiThioalkalivibrio sulfidiphilus (strain HL-EbGR7)
    Taxonomic identifieri396588 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeThioalkalivibrio
    ProteomesiUP000002383: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 693693Polyribonucleotide nucleotidyltransferasePRO_1000185760Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

    Protein-protein interaction databases

    STRINGi396588.Tgr7_1007.

    Structurei

    3D structure databases

    ProteinModelPortaliB8GP06.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini553 – 61260KHUniRule annotationAdd
    BLAST
    Domaini622 – 69069S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8GP06-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAIKKSFQY GQHTVTFETG EIARQASGAV LVNMADTVVL VTAVGLKDVA    50
    QGRDFFPLTV NYQERTYAAG RIPGGFFKRE GRPTEKETLT SRLIDRPIRP 100
    LFPKGFMNEV QVIATVMSMN PEVDPDVPAM LGASAALALS GLPFKGPIGA 150
    ARVGYLNGAY VLNPSMSELK HSDLDLVVAG TEKAVLMVES EAKLLSEEVM 200
    LGAVMYGHEQ MQAAIKVINE LAAEAGKPAW DWVAPEENVA LKEAVAAACE 250
    AELTAAYQIA EKQARNQQVN ALRDAVVAKL ATGEEGAPSA EKVKGAFGAL 300
    EKRIVRSRVL KGEPRIDGRD TRTVRPITVK TGVLPRTHGS ALFTRGETQA 350
    IVVATLGTDR DAQLIEAIEG ERRERFMLHY NFPPFCTGET GMVGSPKRRE 400
    IGHGRLAKRG VQAVMPGEAE CPYVLRVVSE ITESNGSSSM ASVCGTSLAL 450
    MDAGVPLKAP VAGIAMGLIK EGDAFAVISD ILGDEDHLGD MDFKVAGSKD 500
    GVSALQMDIK IDGITREIME KALAQAKEGR LHILEKMNAV LSEPRTEVSA 550
    YAPRFTTLKI HPDKIRDVIG KGGATIRALT EETGTSIDIS DDGTVKIASV 600
    DKAAGDEARR RIEELTADVE VGRIYEGRVV KIMDFGAFVT ILPGRDGLVH 650
    ISQISEERVE SVSDRLTEGD LVKVKVLEVD KQGRIRLSMK EVG 693
    Length:693
    Mass (Da):74,585
    Last modified:March 3, 2009 - v1
    Checksum:iCFA8EC5600653AD8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001339 Genomic DNA. Translation: ACL72095.1.
    RefSeqiYP_002513082.1. NC_011901.1.

    Genome annotation databases

    EnsemblBacteriaiACL72095; ACL72095; Tgr7_1007.
    GeneIDi7316584.
    KEGGitgr:Tgr7_1007.
    PATRICi23961528. VBIThiSp19295_1012.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001339 Genomic DNA. Translation: ACL72095.1 .
    RefSeqi YP_002513082.1. NC_011901.1.

    3D structure databases

    ProteinModelPortali B8GP06.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 396588.Tgr7_1007.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL72095 ; ACL72095 ; Tgr7_1007 .
    GeneIDi 7316584.
    KEGGi tgr:Tgr7_1007.
    PATRICi 23961528. VBIThiSp19295_1012.

    Phylogenomic databases

    eggNOGi COG1185.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci TSUL396588:GH5B-1021-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HL-EbGR7.

    Entry informationi

    Entry nameiPNP_THISH
    AccessioniPrimary (citable) accession number: B8GP06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3