B8GN06 (TYSY_THISH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Tgr7_2747

Organism

Thioalkalivibrio sp. (strain HL-EbGR7) [Complete proteome] [HAMAP]

Taxonomic identifier

396588 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Thioalkalivibrio › Thioalkalivibrio sulfidophilus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Thymidylate synthase HAMAP-Rule MF_00008		PRO_1000197266
Sites
	Active site	159	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8GN06 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 456CC3569E569294	FASTA	277	31,776
	10 20 30 40 50 60 MQPYLDLMRQ LLEHGTVKSD RTGTGTRSLF GHQMRFDLSK GFPLVTTKKL HLKSIIHELL 70 80 90 100 110 120 WFLKGETNIA YLKDNGVRIW DEWATEDGEL GPVYGRQWRA WPTPDGRHID QISQVVEQIR 130 140 150 160 170 180 TRPDSRRLIV SAWNVAELPD EGISPKDNAR AGRMALAPCH TFFQFYVADG RLSCQLYQRS 190 200 210 220 230 240 ADVFLGVPFN IASYALLTLM VAQVTDLEPG DFVHTFGDVH LYSNHVEQAK EQLSREPYPL 250 260 270 PKMHLNPEVK SLFDFRYEDF TLEGYQAHPH IKAPVAI

References

[1]

"Complete genome sequence of 'Thioalkalivibrio sulfidophilus' HL-EbGr7." Muyzer G., Sorokin D.Y., Mavromatis K., Lapidus A., Clum A., Ivanova N., Pati A., d'Haeseleer P., Woyke T., Kyrpides N.C. Stand. Genomic Sci. 4:23-35(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HL-EbGR7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001339 Genomic DNA. Translation: ACL73821.1.
RefSeq	YP_002514808.1. NC_011901.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	396588.Tgr7_2747.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL73821; ACL73821; Tgr7_2747.
GeneID	7316909.
KEGG	tgr:Tgr7_2747.
PATRIC	23964998. VBIThiSp19295_2720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
KO	K00560.
OMA	DTNVAYL.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_THISH

Accession

Primary (citable) accession number: B8GN06

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program