B8GJQ5 (B8GJQ5_METPE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydromethanopterin reductase HAMAP MF_01091
EC=1.5.99.11 HAMAP MF_01091

Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase HAMAP MF_01091
Methylene-H(4)MPT reductase HAMAP MF_01091

Gene names

Name:	mer HAMAP MF_01091
Ordered Locus Names:	Mpal_0327

Organism

Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]

Taxonomic identifier

521011 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT By similarity. HAMAP MF_01091
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_01091
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP MF_01091
Subcellular location	Cytoplasm By similarity HAMAP MF_01091.
Sequence similarities	Belongs to the mer family. HAMAP MF_01091

Ontologies

Keywords
Biological process	Methanogenesis HAMAP MF_01091 One-carbon metabolism HAMAP MF_01091
Cellular component	Cytoplasm HAMAP MF_01091
Molecular function	Oxidoreductase HAMAP MF_01091 EMBL ACL15709.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: HAMAP one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B8GJQ5 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 1A85F1D54AB7AC49	FASTA	328	34,799
	10 20 30 40 50 60 MTYGIEFVPG NVNVKQVVKF CKLAESKDID FAWITNHYNN RHCYPVLAAI AQATDSIKMG 70 80 90 100 110 120 PGIMNSFTDT PAAMASFMAT LNEISDGRAV LGIGPGDLST LPKLAIQASK PVGHLEEAVV 130 140 150 160 170 180 QIRKLLAGEE IKKSGMQFFD YDGAKLTGVT LPGKKGIPVY IGAQGPKVLE LAGTIGDGAL 190 200 210 220 230 240 INASNPKDFA VAIPIIKAAN EKVGKKKFDI GAYTAMSIDM NEKKARNAAK IVAAFIAAGS 250 260 270 280 290 300 PEPLLTRHGL DLDNVAKIKA ALGKFDFKSV GELVGDKEID AFTIAGTPDM VKQKCEDLTK 310 320 AGVTQIIFGS PLGPDMTTSI RLLGKYVV

References

[1]

"Complete sequence of Candidatus Methanosphaerula E1-9c." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001338 Genomic DNA. Translation: ACL15709.1.
RefSeq	YP_002465432.1. NC_011832.1.
3D structure databases
ProteinModelPortal	B8GJQ5.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GJQ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7272628.
GenomeReviews	Gene locus Mpal_0327 in contig CP001338_GR.
KEGG	mpl:Mpal_0327.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497169.
OMA	GIEFVPG.
ProtClustDB	PRK02271.
Family and domain databases
HAMAP	MF_01091. F420_mer. [Tree]
InterPro	IPR011251. Luciferase-like_dom. IPR019946. MeH4methanopterin_reductase. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
KO	K00320.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
SUPFAM	SSF51679. Luciferase_like. 1 hit.
TIGRFAMs	TIGR03555. F420_mer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8GJQ5_METPE

Accession

Primary (citable) accession number: B8GJQ5

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)