B8GJJ4 (B8GJJ4_METPE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA reductase HAMAP MF_00087
Short name=GluTR HAMAP MF_00087
EC=1.2.1.70 HAMAP MF_00087

Gene names

Name:	hemA HAMAP MF_00087
Ordered Locus Names:	Mpal_1728

Organism

Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]

Taxonomic identifier

521011 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA) By similarity. HAMAP MF_00087
Catalytic activity	L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH. HAMAP MF_00087 RuleBase RU000584
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2. HAMAP MF_00087 RuleBase RU000584
Subunit structure	Homodimer By similarity. HAMAP MF_00087
Domain	Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization By similarity. HAMAP MF_00087
Miscellaneous	During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity. HAMAP MF_00087
Sequence similarities	Belongs to the glutamyl-tRNA reductase family. HAMAP MF_00087 RuleBase RU000584

Ontologies

Keywords
Biological process	Porphyrin biosynthesis HAMAP MF_00087 RuleBase RU000584
Ligand	NADP HAMAP MF_00087 RuleBase RU000584
Molecular function	Oxidoreductase HAMAP MF_00087 RuleBase RU000584 EMBL ACL17035.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamyl-tRNA reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	180 – 185	6	NADP By similarity HAMAP MF_00087
	Region	52 – 55	4	Substrate binding By similarity HAMAP MF_00087
	Region	105 – 107	3	Substrate binding By similarity HAMAP MF_00087
Sites
	Active site	53	1	Nucleophile By similarity HAMAP MF_00087
	Binding site	100	1	Substrate By similarity HAMAP MF_00087
	Binding site	111	1	Substrate By similarity HAMAP MF_00087
	Site	90	1	Important for activity By similarity HAMAP MF_00087

Sequences

	Sequence		Length	Mass (Da)
	B8GJJ4 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: BB53CECED71571F3	FASTA	425	46,559
	10 20 30 40 50 60 MAVDPLFAHI AIGGMNHHTA DMDMLEQFRF GDEEQFLADI QVHFKGAILL QTCNRVEILV 70 80 90 100 110 120 QGTGAGLTEY LHELGRASFE VREDLDAIRH LLEVACGIDS MIVGEDQILG QLKKALAAAQ 130 140 150 160 170 180 TAGACSPLIE QCVNKAVHVG VEVRRRTEIN RGAVSIGSAA VTLAEELLGT LTGRHILVVG 190 200 210 220 230 240 GGEIGMLVTQ ALAAKDLTAI YVANRTYARA VQLASKIGGK AVTMAEMHRY LVLSDVVISC 250 260 270 280 290 300 TSAPHPIIRC EELKEVMKGR CWPLEGHPRP LILIDIAQPR DVEVGADQID GVHLCTIDNL 310 320 330 340 350 360 RTISQNNLDY RKSEASRAQT YIEEELNQFV QLINRKAADD HLSMLHSWAE QIRVRERDRA 370 380 390 400 410 420 LARLGSEDQR AVAVVDDLTR VLVKKLLTDA TFSIRASAEC GEMEAAASLV HAITQGEKLC IQKKE

References

[1]

"Complete sequence of Candidatus Methanosphaerula E1-9c." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001338 Genomic DNA. Translation: ACL17035.1.
RefSeq	YP_002466758.1. NC_011832.1.
3D structure databases
ProteinModelPortal	B8GJJ4.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GJJ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7271291.
GenomeReviews	Gene locus Mpal_1728 in contig CP001338_GR.
KEGG	mpl:Mpal_1728.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG732626.
OMA	GPILNRL.
ProtClustDB	PRK00045.
Family and domain databases
HAMAP	MF_00087. Glu_tRNA_reductase. [Tree]
InterPro	IPR000343. 4pyrrol_synth_GluRdtase. IPR015896. 4pyrrol_synth_GluRdtase_dimer. IPR015895. 4pyrrol_synth_GluRdtase_N. IPR016040. NAD(P)-bd_dom. IPR018214. Pyrrol_synth_GluRdtase_CS. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K02492.
Pfam	PF00745. GlutR_dimer. 1 hit. PF05201. GlutR_N. 1 hit. PF01488. Shikimate_DH. 1 hit. [Graphical view]
PIRSF	PIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
SUPFAM	SSF69075. 4pyrrol_synth_GluRdtase_C. 1 hit. SSF69742. GlutR. 1 hit.
TIGRFAMs	TIGR01035. HemA. 1 hit.
PROSITE	PS00747. GLUTR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8GJJ4_METPE

Accession

Primary (citable) accession number: B8GJJ4

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)