B8GJJ3 (B8GJJ3_METPE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase RuleBase RU000515
EC=4.2.1.24 RuleBase RU000515

Gene names

Ordered Locus Names:

Mpal_1727

Organism

Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]

Taxonomic identifier

521011 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O. RuleBase RU000515
Subunit structure	Homooctamer By similarity. RuleBase RU000515 PIRSR PIRSR001415-3
Sequence similarities	Belongs to the ALADH family. RuleBase RU004161

Ontologies

Keywords
Biological process	Porphyrin biosynthesis RuleBase RU000515
Ligand	Magnesium PIRSR PIRSR001415-5 Metal-binding PIRSR PIRSR001415-5 Zinc PIRSR PIRSR001415-3
Molecular function	Lyase RuleBase RU000515 EMBL ACL17034.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	192	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Active site	245	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Metal binding	112	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	114	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	122	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	230	1	Magnesium By similarity PIRSR PIRSR001415-5
	Binding site	202	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	214	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	270	1	Substrate 2 By similarity PIRSR PIRSR001415-2
	Binding site	309	1	Substrate 2 By similarity PIRSR PIRSR001415-2

Sequences

	Sequence		Length	Mass (Da)
	B8GJJ3 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 3C0CB66C399A44A6	FASTA	323	34,790
	10 20 30 40 50 60 MRRLRGRHLL PLLTEHHLNK KDLIAPIFVD QTITVPRPIE TMPGQNRYPV SAVGEVATRL 70 80 90 100 110 120 FRKGIWALLL FGVPEVKDPE ASGAFDPNGV IQQAVRAVKQ AVPAMVVLTD VCACEYTSSG 130 140 150 160 170 180 ICGIGGPNRC GEVDLLNDPS LALMADIAVS HAEAGADIVA PSCMLDGMVI TIRHALDQAG 190 200 210 220 230 240 FQDVLILSYS TKFASALYGP FRDAADSSCF CGDRTSYQIN PANGREGYME SRLDVDEGAD 250 260 270 280 290 300 MLMVKPAGFY LDVLASITDF GLPVAAYQVS GEYALIMAAA ENGWINKKAV AMESLIAIKR 310 320 AGADLIITYF AEDVAGWLDE DCE

References

[1]

"Complete sequence of Candidatus Methanosphaerula E1-9c." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001338 Genomic DNA. Translation: ACL17034.1.
RefSeq	YP_002466757.1. NC_011832.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8GJJ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7271290.
GenomeReviews	Gene locus Mpal_1727 in contig CP001338_GR.
KEGG	mpl:Mpal_1727.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285270.
OMA	ADLCFCE.
ProtClustDB	PRK09283.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01698.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8GJJ3_METPE

Accession

Primary (citable) accession number: B8GJJ3

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)