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B8GJF9 (THIM_METPE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:Mpal_1692
OrganismMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]
Taxonomic identifier521011 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanosphaerula

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383920

Sites

Binding site441Substrate; via amide nitrogen By similarity
Binding site1201ATP By similarity
Binding site1661ATP By similarity
Binding site1931Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B8GJF9 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 4CDBEFA2044044D5

FASTA26526,613
        10         20         30         40         50         60 
MTLDLASALN DLQTKKPLVH HITNAVTIND CANITLCIGA APVMAEAPEE VAEMVAMAGA 

        70         80         90        100        110        120 
LVLNIGTLSK SQISAMLIAG RAANDLEVPV ILDPVGAGAT MLRTESARRL LTELQVAVVK 

       130        140        150        160        170        180 
GNAGEIGLLA GAAGRVRGVD SAGLDGDPVA VCKSLVNAYS CTVAMSGATD IVTDGDRVVL 

       190        200        210        220        230        240 
IDNGDPRMGV VSGTGCMASS LTGAFAAGSR DQVCSTAAAL AAFGLAGEKA AKRAFGPSSF 

       250        260 
KVALMDEVAA LTPPVLSAGA KIRIL 

« Hide

References

[1]"Complete sequence of Candidatus Methanosphaerula E1-9c."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001338 Genomic DNA. Translation: ACL17000.1.
RefSeqYP_002466723.1. NC_011832.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING521011.Mpal_1692.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL17000; ACL17000; Mpal_1692.
GeneID7271255.
KEGGmpl:Mpal_1692.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAVSGTGCM.

Enzyme and pathway databases

BioCycMPAL521011:GI2F-1737-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_METPE
AccessionPrimary (citable) accession number: B8GJF9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 3, 2009
Last modified: June 11, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways