SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B8GJ69

- B8GJ69_METPE

UniProt

B8GJ69 - B8GJ69_METPE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ribulose bisphosphate carboxylase

Gene
rbcL, Mpal_0258
Organism
Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase By similarity.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381Proton acceptor By similarityUniRule annotation
Binding sitei140 – 1401Substrate By similarityUniRule annotation
Metal bindingi164 – 1641Magnesium; via carbamate group By similarityUniRule annotation
Metal bindingi166 – 1661Magnesium By similarityUniRule annotation
Metal bindingi167 – 1671Magnesium By similarityUniRule annotation
Active sitei255 – 2551Proton acceptor By similarityUniRule annotation
Binding sitei256 – 2561Substrate By similarityUniRule annotation
Binding sitei288 – 2881Substrate By similarityUniRule annotation
Sitei295 – 2951Transition state stabilizer By similarityUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbon fixation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciMPAL521011:GI2F-262-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:Mpal_0258Imported
OrganismiMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)Imported
Taxonomic identifieri521011 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanosphaerula
ProteomesiUP000002457: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei164 – 1641N6-carboxylysine By similarityUniRule annotation

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi521011.Mpal_0258.

Structurei

3D structure databases

ProteinModelPortaliB8GJ69.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 3273Substrate binding By similarityUniRule annotation
Regioni347 – 3504Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Belongs to the RuBisCO large chain family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1850.
HOGENOMiHOG000230832.
KOiK01601.
OMAiVIVTFRV.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
InterProiIPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.

Sequencei

Sequence statusi: Complete.

B8GJ69-1 [UniParc]FASTAAdd to Basket

« Hide

MSDVTAIYYF RPRADTSPEQ AAQAICEEET TGTWTDISTT TEYVRRLDGA    50
VESVEPKGQG YLTKITYPAE IFEPGNVPQY LSVVAGNLFG LGRVEAVRLL 100
DVEFPRELVT FSGPKFGIDG VRRLVGSQNR PHVGTIIKPK VGLNPTDTAE 150
VAYQAAIGGV DLIKDDETLT DQQFCPLFER LPAVMARLDQ AKTETGRNVL 200
YAVNVTTRAD KILERAEHAL ELGANMLMVD VISAGFSAVQ ALAEDPSIKV 250
PIHVHRTMHA AMTRNPEHGI AMRTFAKLVR MLGGDQLHTG SVSGKMSHDA 300
EELLGDNRAL TDQCYGLKST FPVSSGGLHP GKVQTELRVL GTDLILQAGG 350
GIHGHPDGTA AGARAMRQAV DAFMEGIPTV EYAKDHYELD RALKKWGTS 399
Length:399
Mass (Da):43,284
Last modified:March 3, 2009 - v1
Checksum:i793E78D3ACFB889C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001338 Genomic DNA. Translation: ACL15642.1.
RefSeqiWP_012616961.1. NC_011832.1.
YP_002465365.1. NC_011832.1.

Genome annotation databases

EnsemblBacteriaiACL15642; ACL15642; Mpal_0258.
GeneIDi7270644.
KEGGimpl:Mpal_0258.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001338 Genomic DNA. Translation: ACL15642.1 .
RefSeqi WP_012616961.1. NC_011832.1.
YP_002465365.1. NC_011832.1.

3D structure databases

ProteinModelPortali B8GJ69.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 521011.Mpal_0258.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL15642 ; ACL15642 ; Mpal_0258 .
GeneIDi 7270644.
KEGGi mpl:Mpal_0258.

Phylogenomic databases

eggNOGi COG1850.
HOGENOMi HOG000230832.
KOi K01601.
OMAi VIVTFRV.

Enzyme and pathway databases

BioCyci MPAL521011:GI2F-262-MONOMER.

Family and domain databases

Gene3Di 3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
InterProi IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view ]
Pfami PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Candidatus Methanosphaerula E1-9c."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Entry informationi

Entry nameiB8GJ69_METPE
AccessioniPrimary (citable) accession number: B8GJ69
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains By similarity.UniRule annotation

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi