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B8GJ69

- B8GJ69_METPE

UniProt

B8GJ69 - B8GJ69_METPE

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei138 – 1381Proton acceptorUniRule annotation
    Binding sitei140 – 1401SubstrateUniRule annotation
    Metal bindingi164 – 1641Magnesium; via carbamate groupUniRule annotation
    Metal bindingi166 – 1661MagnesiumUniRule annotation
    Metal bindingi167 – 1671MagnesiumUniRule annotation
    Active sitei255 – 2551Proton acceptorUniRule annotation
    Binding sitei256 – 2561SubstrateUniRule annotation
    Binding sitei288 – 2881SubstrateUniRule annotation
    Sitei295 – 2951Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: InterPro

    Keywords - Molecular functioni

    LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciMPAL521011:GI2F-262-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    Ordered Locus Names:Mpal_0258Imported
    OrganismiMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)Imported
    Taxonomic identifieri521011 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanosphaerula
    ProteomesiUP000002457: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei164 – 1641N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi521011.Mpal_0258.

    Structurei

    3D structure databases

    ProteinModelPortaliB8GJ69.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni325 – 3273Substrate bindingUniRule annotation
    Regioni347 – 3504Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family.UniRule annotation
    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1850.
    HOGENOMiHOG000230832.
    KOiK01601.
    OMAiVIVTFRV.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProiIPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B8GJ69-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDVTAIYYF RPRADTSPEQ AAQAICEEET TGTWTDISTT TEYVRRLDGA    50
    VESVEPKGQG YLTKITYPAE IFEPGNVPQY LSVVAGNLFG LGRVEAVRLL 100
    DVEFPRELVT FSGPKFGIDG VRRLVGSQNR PHVGTIIKPK VGLNPTDTAE 150
    VAYQAAIGGV DLIKDDETLT DQQFCPLFER LPAVMARLDQ AKTETGRNVL 200
    YAVNVTTRAD KILERAEHAL ELGANMLMVD VISAGFSAVQ ALAEDPSIKV 250
    PIHVHRTMHA AMTRNPEHGI AMRTFAKLVR MLGGDQLHTG SVSGKMSHDA 300
    EELLGDNRAL TDQCYGLKST FPVSSGGLHP GKVQTELRVL GTDLILQAGG 350
    GIHGHPDGTA AGARAMRQAV DAFMEGIPTV EYAKDHYELD RALKKWGTS 399
    Length:399
    Mass (Da):43,284
    Last modified:March 3, 2009 - v1
    Checksum:i793E78D3ACFB889C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001338 Genomic DNA. Translation: ACL15642.1.
    RefSeqiWP_012616961.1. NC_011832.1.
    YP_002465365.1. NC_011832.1.

    Genome annotation databases

    EnsemblBacteriaiACL15642; ACL15642; Mpal_0258.
    GeneIDi7270644.
    KEGGimpl:Mpal_0258.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001338 Genomic DNA. Translation: ACL15642.1 .
    RefSeqi WP_012616961.1. NC_011832.1.
    YP_002465365.1. NC_011832.1.

    3D structure databases

    ProteinModelPortali B8GJ69.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 521011.Mpal_0258.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL15642 ; ACL15642 ; Mpal_0258 .
    GeneIDi 7270644.
    KEGGi mpl:Mpal_0258.

    Phylogenomic databases

    eggNOGi COG1850.
    HOGENOMi HOG000230832.
    KOi K01601.
    OMAi VIVTFRV.

    Enzyme and pathway databases

    BioCyci MPAL521011:GI2F-262-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProi IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Candidatus Methanosphaerula E1-9c."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S.
      Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1556 / DSM 19958 / E1-9cImported.

    Entry informationi

    Entry nameiB8GJ69_METPE
    AccessioniPrimary (citable) accession number: B8GJ69
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 3, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3