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B8GJ69

- B8GJ69_METPE

UniProt

B8GJ69 - B8GJ69_METPE

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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Note: Binds 1 magnesium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381Proton acceptorUniRule annotation
Binding sitei140 – 1401SubstrateUniRule annotation
Metal bindingi164 – 1641Magnesium; via carbamate groupUniRule annotation
Metal bindingi166 – 1661MagnesiumUniRule annotation
Metal bindingi167 – 1671MagnesiumUniRule annotation
Active sitei255 – 2551Proton acceptorUniRule annotation
Binding sitei256 – 2561SubstrateUniRule annotation
Binding sitei288 – 2881SubstrateUniRule annotation
Sitei295 – 2951Transition state stabilizerUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. oxidoreductase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. AMP catabolic process Source: UniProtKB-HAMAP
  2. carbon fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciMPAL521011:GI2F-262-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:Mpal_0258Imported
OrganismiMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)Imported
Taxonomic identifieri521011 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanosphaerula
ProteomesiUP000002457: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei164 – 1641N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi521011.Mpal_0258.

Structurei

3D structure databases

ProteinModelPortaliB8GJ69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 3273Substrate bindingUniRule annotation
Regioni347 – 3504Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the RuBisCO large chain family.UniRule annotation
Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1850.
HOGENOMiHOG000230832.
KOiK01601.
OMAiVIVTFRV.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
InterProiIPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.

Sequencei

Sequence statusi: Complete.

B8GJ69-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDVTAIYYF RPRADTSPEQ AAQAICEEET TGTWTDISTT TEYVRRLDGA
60 70 80 90 100
VESVEPKGQG YLTKITYPAE IFEPGNVPQY LSVVAGNLFG LGRVEAVRLL
110 120 130 140 150
DVEFPRELVT FSGPKFGIDG VRRLVGSQNR PHVGTIIKPK VGLNPTDTAE
160 170 180 190 200
VAYQAAIGGV DLIKDDETLT DQQFCPLFER LPAVMARLDQ AKTETGRNVL
210 220 230 240 250
YAVNVTTRAD KILERAEHAL ELGANMLMVD VISAGFSAVQ ALAEDPSIKV
260 270 280 290 300
PIHVHRTMHA AMTRNPEHGI AMRTFAKLVR MLGGDQLHTG SVSGKMSHDA
310 320 330 340 350
EELLGDNRAL TDQCYGLKST FPVSSGGLHP GKVQTELRVL GTDLILQAGG
360 370 380 390
GIHGHPDGTA AGARAMRQAV DAFMEGIPTV EYAKDHYELD RALKKWGTS
Length:399
Mass (Da):43,284
Last modified:March 3, 2009 - v1
Checksum:i793E78D3ACFB889C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001338 Genomic DNA. Translation: ACL15642.1.
RefSeqiYP_002465365.1. NC_011832.1.

Genome annotation databases

EnsemblBacteriaiACL15642; ACL15642; Mpal_0258.
GeneIDi7270644.
KEGGimpl:Mpal_0258.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001338 Genomic DNA. Translation: ACL15642.1 .
RefSeqi YP_002465365.1. NC_011832.1.

3D structure databases

ProteinModelPortali B8GJ69.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 521011.Mpal_0258.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL15642 ; ACL15642 ; Mpal_0258 .
GeneIDi 7270644.
KEGGi mpl:Mpal_0258.

Phylogenomic databases

eggNOGi COG1850.
HOGENOMi HOG000230832.
KOi K01601.
OMAi VIVTFRV.

Enzyme and pathway databases

BioCyci MPAL521011:GI2F-262-MONOMER.

Family and domain databases

Gene3Di 3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
InterProi IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view ]
Pfami PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Candidatus Methanosphaerula E1-9c."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1556 / DSM 19958 / E1-9cImported.

Entry informationi

Entry nameiB8GJ69_METPE
AccessioniPrimary (citable) accession number: B8GJ69
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: November 26, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3