B8GIC5 (ILVC_METPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Mpal_0082

Organism

Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]

Taxonomic identifier

521011 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000191025
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	B8GIC5 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 2771B306CD76A862	FASTA	331	36,429
	10 20 30 40 50 60 MIKKYYESDA DLAAVQGKQI AVIGYGSQGR GQALNLRDSG LSVIIGLRPG RSFQQASEDG 70 80 90 100 110 120 FEVFPVADAV KKADIIQILL PDESQGAVYK TEIRPYLADK KCLMFSHGFN IHYGQIVPPP 130 140 150 160 170 180 NVDVVMVAPK GPGHMVRRTF EEGKGVPALI AIEQDYTGDA QAIALGYAKG IGATRAVVFE 190 200 210 220 230 240 TTFREETETD LFGEQAVLCG GVTSLIKAGF ETLVDAGYAP EMAYLEVLHE MKLIVDMIYE 250 260 270 280 290 300 GGFTNMRDSI SNTALYGDLT RGPRVIGEDS RIAMEEILEE IQNGEFAREW MLENIVNRPV 310 320 330 FTALKRNDEE HLLEEVGAEI RGLMPQFKKN A

References

[1]

"Complete sequence of Candidatus Methanosphaerula E1-9c." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001338 Genomic DNA. Translation: ACL15476.1.
RefSeq	YP_002465199.1. NC_011832.1.
3D structure databases
ProteinModelPortal	B8GIC5.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GIC5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7272252.
GenomeReviews	Gene locus Mpal_0082 in contig CP001338_GR.
KEGG	mpl:Mpal_0082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286525.
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_METPE

Accession

Primary (citable) accession number: B8GIC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program