B8GIB2 (GUAAB_METPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	Mpal_0069

Organism

Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]

Taxonomic identifier

521011 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW asparagine biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_1000133394
Regions
	Domain	2 – 184	183	GMPS ATP-PPase
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8GIB2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 4DA8222FD003A153	FASTA	305	33,478
	10 20 30 40 50 60 MVNTERFIQQ AVSEIRELTA DARVVMALSG GVDSSVCAAL AAQAIGERLE PIYVDTGLMR 70 80 90 100 110 120 KGETDRIRHL FGHLNLRIVD ASDEFLDALA GVADPETKRK IIGERFIRVF EREAEKTGAT 130 140 150 160 170 180 VLLQGTIYPD RIESEGGIKS HHNVGGMPLG IAFTQVLEPL RDLYKDEVRE VAGALGLPRE 190 200 210 220 230 240 IQHRMPFPGP GLAVRIIGEV TREKVAVIRE ANAITEEELV EEFRPWQCLA ALVGLGTGVK 250 260 270 280 290 300 GDIRLHGWIV AVRAVNSRDG MTADPLEVPY PVLFKIASRI TAEIPSVARV VYDVTPKPPA TIEYE

References

[1]

"Complete sequence of Candidatus Methanosphaerula E1-9c." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001338 Genomic DNA. Translation: ACL15463.1.
RefSeq	YP_002465186.1. NC_011832.1.
3D structure databases
ProteinModelPortal	B8GIB2.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GIB2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7272238.
GenomeReviews	Gene locus Mpal_0069 in contig CP001338_GR.
KEGG	mpl:Mpal_0069.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	QGTIAPD.
ProtClustDB	PRK00919.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001962. Asn_synthase. IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00733. Asn_synthase. 1 hit. PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_METPE

Accession

Primary (citable) accession number: B8GIB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program