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Protein

dCTP deaminase, dUMP-forming

Gene

dcd

Organism
Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate.UniRule annotation

Catalytic activityi

dCTP + 2 H2O = dUMP + diphosphate + NH3.UniRule annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dUMP from dCTP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. dCTP deaminase, dUMP-forming (dcd)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP, the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei114 – 115Important for bifunctional activityUniRule annotation2
Binding sitei117dCTPUniRule annotation1
Active sitei127Proton donor/acceptorUniRule annotation1
Binding sitei146dCTPUniRule annotation1
Binding sitei159dCTPUniRule annotation1
Binding sitei166dCTPUniRule annotation1
Binding sitei170dCTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi99 – 104dCTP bindingUniRule annotation6
Nucleotide bindingi125 – 127dCTP bindingUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciMPAL521011:G1GUJ-1330-MONOMER
UniPathwayiUPA00610; UER00667

Names & Taxonomyi

Protein namesi
Recommended name:
dCTP deaminase, dUMP-formingUniRule annotation (EC:3.5.4.30UniRule annotation)
Alternative name(s):
Bifunctional dCTP deaminase:dUTPaseUniRule annotation
DCD-DUTUniRule annotation
Gene namesi
Name:dcdUniRule annotation
Ordered Locus Names:Mpal_1302
OrganismiMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)
Taxonomic identifieri521011 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanosphaerula
Proteomesi
  • UP000002457 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001231511 – 186dCTP deaminase, dUMP-formingAdd BLAST186

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi521011.Mpal_1302

Structurei

3D structure databases

ProteinModelPortaliB8GHN1
SMRiB8GHN1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the dCTP deaminase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04048 Archaea
COG0717 LUCA
HOGENOMiHOG000228601
KOiK01494
OMAiGWIDAGF
OrthoDBiPOG093Z0C6C

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.10, 1 hit
HAMAPiMF_00146 dCTP_deaminase, 1 hit
InterProiView protein in InterPro
IPR011962 dCTP_deaminase
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR02274 dCTP_deam, 1 hit

Sequencei

Sequence statusi: Complete.

B8GHN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILVDWQIMD RISRGQIKVE PFEPGLIQPN SLDIRLGDHF VWYVPGDRVI
60 70 80 90 100
DPYDKESVTA EVEEMNANEV ILYPGQFMLA ETKEVLSLPD NVVASIEGKS
110 120 130 140 150
SIARLGIELH QTGGWIDAGF IGSITLEMCN VNQRPVKMYA GMPIGQLVFY
160 170 180
TTERAEQPYN LKQDAKYQGQ RQATLSRYHE NQRFIQ
Length:186
Mass (Da):21,206
Last modified:March 3, 2009 - v1
Checksum:iB3E661C1BFC7F1E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001338 Genomic DNA Translation: ACL16636.1
RefSeqiWP_012617955.1, NC_011832.1

Genome annotation databases

EnsemblBacteriaiACL16636; ACL16636; Mpal_1302
GeneIDi7271162
KEGGimpl:Mpal_1302

Similar proteinsi

Entry informationi

Entry nameiDCDB_METPE
AccessioniPrimary (citable) accession number: B8GHN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 25, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health