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B8GHL6 (B8GHL6_METPE) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alanine--tRNA ligase HAMAP MF_00036
EC=6.1.1.7 HAMAP MF_00036
Alternative name(s):
Alanyl-tRNA synthetase HAMAP MF_00036
Gene names
Name:alaS HAMAP MF_00036
Ordered Locus Names:Mpal_1287
OrganismMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]
Taxonomic identifier521011 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length913 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036

Catalytic activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036 SAAS SAAS022429

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036 SAAS SAAS022429

Subcellular location

Cytoplasm By similarity HAMAP MF_00036.

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP MF_00036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6081Zinc By similarity HAMAP MF_00036
Metal binding6121Zinc By similarity HAMAP MF_00036
Metal binding7111Zinc By similarity HAMAP MF_00036
Metal binding7151Zinc By similarity HAMAP MF_00036

Sequences

Sequence LengthMass (Da)Tools
B8GHL6 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: ABD2C3F6FE2C059B

FASTA913101,894
        10         20         30         40         50         60 
MSEEDYELDY FKSEGLVRKT CTSCGASFWT RDPERQVCGD APCEPYNFIG NPVFRPHTLD 

        70         80         90        100        110        120 
EMREAYLSFF ERHGHTRIDR YPVAARWRDD IYLTIASIAD FQPYVTSGVV PPPANPLTIS 

       130        140        150        160        170        180 
QPCIRLNDLD SVGRSGRHLT LFEMMAHHAF NSPKEEIYWK DHTVELCDEF VHSIGGDLNA 

       190        200        210        220        230        240 
ITYKEHPWIG GGNAGPSVEV MIGGLEIATL VFMSLGRQKT DHPPVELEGN QYYPMSTRVV 

       250        260        270        280        290        300 
DTGYGLERMV WASTGSPTIY DAVFPDMVST IMHSAGLDHH LDNPESTKML ALNAKFAGLM 

       310        320        330        340        350        360 
DISGANLFQL RKKVAATIDV PIEKLQKMIV PIEQVYTIAD HTRCLAYMLG DLIVPSNVRE 

       370        380        390        400        410        420 
GYLARLVLRR TLRVMNDLKM DIDLGDLIEM QINKIGNSQF EQNLDVIHEI VDREVEKYAA 

       430        440        450        460        470        480 
TIERGTRIIQ KVATTYKKKS EKVPLQEVVT LYDSHGLPPE LIKEVASKEG AVVDLPDNFY 

       490        500        510        520        530        540 
SLIADMHSES QKEQVDDPLE SYRERVSGLP DTHPLYYELP TEVEFEGMVL DFFDGYVILD 

       550        560        570        580        590        600 
QTLFYPEGGG QPADTGTLVT AESMVRVEDT IKMGGVILHK ISGGSLKRGE RVKGMVDEDR 

       610        620        630        640        650        660 
RWSLMRHHTA THILLHASKE VLGAHIHQSG AQKGSESSRI DIRHFKHITP EELRRIEVKA 

       670        680        690        700        710        720 
NQIVMKNRPI SIKIEERTKA EQKYGFGLYQ GGVPPGKEIR IVKVDGDIEA CAGTHCKSTG 

       730        740        750        760        770        780 
EVGAIKILRV EHIQDGIERI EFSAGRAAIT AMQHVEQLLD ESAQTLRIQP ENLPSSVQRF 

       790        800        810        820        830        840 
FTEWKERGKE VERLRARLVD LELQNVRYES VRGVNLVIKE IDVDPSQLAS LARKLSKNDG 

       850        860        870        880        890        900 
VALLASSTDT LHLVLSSGSR SVDAGKILGQ IALLIDGKGG GNATMAHGIS EDRSQLEPAL 

       910 
QAGKKLILAS LGA

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References

[1]"Complete sequence of Candidatus Methanosphaerula E1-9c."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001338 Genomic DNA. Translation: ACL16621.1.
RefSeqYP_002466344.1. NC_011832.1.

3D structure databases

ProteinModelPortalB8GHL6.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8GHL6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7271147.
GenomeReviewsGene locus Mpal_1287 in contig CP001338_GR.
KEGGmpl:Mpal_1287.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG392147.
OMAMFTNSGM.
ProtClustDBPRK13902.

Family and domain databases

HAMAPMF_00036_A. Ala_tRNA_synth_A.
[Tree]
InterProIPR002318. Ala-tRNA-synth_IIc.
IPR018162. Ala-tRNA-synth_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR022429. Ala-tRNA_synth_arc.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
KOK01872.
PANTHERPTHR11777:SF6. PTHR11777:SF6. 1 hit.
PfamPF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR00980. TRNASYNTHALA.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR03683. A-tRNA_syn_arch. 1 hit.
TIGR00344. AlaS. 1 hit.
PROSITEPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB8GHL6_METPE
AccessionPrimary (citable) accession number: B8GHL6
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info