B8GH19 (ARLY_METPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate lyase
Short name=ASAL
EC=4.3.2.1

Alternative name(s):
Arginosuccinase

Gene names

Name:	argH
Ordered Locus Names:	Mpal_1078

Organism

Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]

Taxonomic identifier

521011 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. HAMAP MF_00006
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3. HAMAP MF_00006
Subcellular location	Cytoplasm By similarity HAMAP MF_00006.
Sequence similarities	Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process via ornithine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	argininosuccinate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Argininosuccinate lyase HAMAP MF_00006		PRO_1000116333

Sequences

	Sequence		Length	Mass (Da)
	B8GH19 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 9A5DC8E09AC638B0	FASTA	494	53,416
	10 20 30 40 50 60 MIHDVVREGR LSGVRSGEVA AFLSSRNADH WIAEADVLVD IAHLLMLYRQ GIIEPEPAAA 70 80 90 100 110 120 VMDVLLRFSA DGVPEEAFDE QFEDIHAGIE SLLIAEVGIE QGGRLHIGRS RNDEVATCLR 130 140 150 160 170 180 LRLCEELLVI GDAVNGLCEV LVSLAGEHTH SVMPGFTHLQ HAQPTTLAHH LLSYAQAFSR 190 200 210 220 230 240 DLERLLSTYQ RVNRSPLGAA AFASTGYPID REFTATMLGF SGVLENSMDA VSTRDFALEA 250 260 270 280 290 300 LADCAILMVN ASRCCEELIL WSSSLVRFVQ LDDAYCSTSS IMPQKKNPDT LEIMRARAGS 310 320 330 340 350 360 VIGASTAALS IVKALPMSYN RDLQQLTPHL WEGIGDAGAS IRILAGALST ATFNTERMET 370 380 390 400 410 420 EAGRGFSTAT ELADMMVREC GLPFRTAHHI VGRAVRSGSL TLVSLKKAAT GESIDLTALG 430 440 450 460 470 480 LTEEKVQAAL DPVHGIEARS VLGGPAVASV STAIETLQES VKAGRTAIAE ERYRRTTAIT 490 TLIKEARRLK TFHQ

References

[1]

"Complete sequence of Candidatus Methanosphaerula E1-9c." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001338 Genomic DNA. Translation: ACL16424.1.
RefSeq	YP_002466147.1. NC_011832.1.
3D structure databases
ProteinModelPortal	B8GH19.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GH19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7270994.
GenomeReviews	Gene locus Mpal_1078 in contig CP001338_GR.
KEGG	mpl:Mpal_1078.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG539632.
OMA	EDIHTVI.
ProtClustDB	PRK00855.
Family and domain databases
HAMAP	MF_00006. Arg_succ_lyase. [Tree]
InterPro	IPR009049. Argininosuccinate_lyase. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR008948. L-Aspartase-like. IPR022761. Lyase1_N. [Graphical view]
KO	K01755.
PANTHER	PTHR11444:SF3. argH. 1 hit.
Pfam	PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00838. ArgH. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLY_METPE

Accession

Primary (citable) accession number: B8GH19

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program