B8GGP2 (B8GGP2_METPE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase HAMAP MF_01571
EC=6.1.1.15 HAMAP MF_01571

Alternative name(s):
Prolyl-tRNA synthetase HAMAP MF_01571

Gene names

Name:	proS HAMAP MF_01571
Ordered Locus Names:	Mpal_0945

Organism

Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]

Taxonomic identifier

521011 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily. HAMAP MF_01571

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_01571
Cellular component	Cytoplasm HAMAP MF_01571
Ligand	ATP-binding HAMAP MF_01571 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_01571 EMBL ACL16297.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B8GGP2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 6DB76ED8D5452996	FASTA	479	54,233
	10 20 30 40 50 60 MQTEEETTAL PPKEEFSAWY NDLLWRAEIM DVRYPVKGVY VWFPFGFSIR KLTYAILRQL 70 80 90 100 110 120 LDKDHQETLF PLLIPEQEFM KEAEHIKGFE EEVYWVTHGG LTELDVKLAL RPTSETAIYP 130 140 150 160 170 180 MYALWVRSHA DLPLKLYQIV NTFRYETKHT RPLIRLREIT SFKEAHTVHA TWDEAAAQVE 190 200 210 220 230 240 DALALYTTFY QHLCVPILIS RRPEWDKFPG ADYTMAVDTV MPDGKTLQIG TVHHLGDHFS 250 260 270 280 290 300 RTFDITYENQ NGEQQLAYQT CYGISERCIA ALISMHGDDK GLILPPEVAP VQVVVVPIIV 310 320 330 340 350 360 GKRRDEVLAA ARTIRDQLEA AGLRVKLDER EIRPGAKYYH WEMRGVPLRV ELGPRDLDNG 370 380 390 400 410 420 VVTAVTRSKE KSTIPAASLI EGVQALLEQV RNTLATTGGQ HLAAHLKQVE TMDDLKVAIE 430 440 450 460 470 EGVAIVHWCG DKACADRIEE ETEASVLGTE VRSSLVSKTE GRCLVCGRAG TTTLIGRSY

References

[1]

"Complete sequence of Candidatus Methanosphaerula E1-9c." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001338 Genomic DNA. Translation: ACL16297.1.
RefSeq	YP_002466020.1. NC_011832.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8GGP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7272438.
GenomeReviews	Gene locus Mpal_0945 in contig CP001338_GR.
KEGG	mpl:Mpal_0945.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG334108.
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8GGP2_METPE

Accession

Primary (citable) accession number: B8GGP2

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)