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B8GGB8 (DNLI_METPE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:Mpal_2781
OrganismMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]
Taxonomic identifier521011 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanosphaerula

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556DNA ligase HAMAP-Rule MF_00407
PRO_1000134728

Sites

Active site2471N6-AMP-lysine intermediate By similarity
Binding site2451ATP By similarity
Binding site2521ATP By similarity
Binding site2671ATP By similarity
Binding site2961ATP By similarity
Binding site3361ATP By similarity
Binding site4081ATP By similarity
Binding site4141ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B8GGB8 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 6665DC65DB81AE57

FASTA55661,297
        10         20         30         40         50         60 
MQFSIFAQTC AALEAQNGRL EMKHAISVIL PSLSGEDLPI FIRFLMGKIF PDWSPQKLGI 

        70         80         90        100        110        120 
GPNLLYEAVA YVAGTKKTAL VDLINRTGDA GLAIEQFLAT KEQTAFFTED PSLAEVYAAC 

       130        140        150        160        170        180 
TRIAASAGGR SQRERLLVLR QLFGNVSPFE ARYLARLILG ELRIGIGEGT VRDAIAEAYT 

       190        200        210        220        230        240 
VEPAQVEHAM QALNDLGEVA LRAREGEEGL IHLSIAPFRP VKMMLAQAGT TIPEMLAAHG 

       250        260        270        280        290        300 
EVAVEFKYDG TRFQFHKEGK TCRIYSRKLE EVTDAVPEVG EALLGATDHD VILDGEVIAI 

       310        320        330        340        350        360 
GADGRPLPFQ TVLRRFRRKH GIAAAREAIT LVPRVFDILY RDGETLIDLP FQSRRAILSA 

       370        380        390        400        410        420 
TIGPEYLAPQ QVLSSAEAVD LLYLEAMAEG HEGVMLKDLL SLYSPGVRGK HWVKIKPEVE 

       430        440        450        460        470        480 
TLDLVVIGAE WGEGRRARTF GSFLLACLDQ GVFRAVSKVA TGISDEQLQE LYTLFKDQVI 

       490        500        510        520        530        540 
AESGNTVTFE PTVIFEVGYA EIQKSPSYES GYALRFPRFV QVRDDKAVEE IETLESLTTR 

       550 
YLAQKTQANG QPEFTL 

« Hide

References

[1]"Complete sequence of Candidatus Methanosphaerula E1-9c."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001338 Genomic DNA. Translation: ACL18036.1.
RefSeqYP_002467759.1. NC_011832.1.

3D structure databases

ProteinModelPortalB8GGB8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING521011.Mpal_2781.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL18036; ACL18036; Mpal_2781.
GeneID7272662.
KEGGmpl:Mpal_2781.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAGRPRPFQ.
ProtClustDBCLSK949919.

Enzyme and pathway databases

BioCycMPAL521011:GI2F-2851-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_METPE
AccessionPrimary (citable) accession number: B8GGB8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families