B8GF03 (PYRB_METPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	Mpal_2536

Organism

Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]

Taxonomic identifier

521011 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Subunit structure	Heterooligomer of catalytic and regulatory chains By similarity.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_1000116150

Sequences

	Sequence		Length	Mass (Da)
	B8GF03 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 755EFA648CD1F53E	FASTA	298	33,265
	10 20 30 40 50 60 MKDFISIRDF DRAAIDRLIA EAERIDHHQY DPLEMKGKIL ALLFFEPSTR TRMSFEAAMA 70 80 90 100 110 120 RLGGTSLTLS SFEASSMAKG ETLADTIRVV SGYVDAIVLR HPREGAARLA SEVSSVPVIN 130 140 150 160 170 180 AGDGAGQHPS QTLLDLYTIR QSMPIDGINI GLLGDLRYGR TTHSLTYALS LYDAVIHTVA 190 200 210 220 230 240 PAGLNLPSGL VHELRELGTE VIEHEQIEDV IKELDVLYVT RIQRERFPDT ASYFNVASSY 250 260 270 280 290 RITPELLRGT KEHMVVLHPL PRVDEIDPRV DKMKNARYFE QSHNGVPVRM AMLKQVIA

References

[1]

"Complete sequence of Candidatus Methanosphaerula E1-9c." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001338 Genomic DNA. Translation: ACL17809.1.
RefSeq	YP_002467532.1. NC_011832.1.
3D structure databases
ProteinModelPortal	B8GF03.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GF03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7271705.
GenomeReviews	Gene locus Mpal_2536 in contig CP001338_GR.
KEGG	mpl:Mpal_2536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	DLYTIRQ.
ProtClustDB	PRK00856.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_METPE

Accession

Primary (citable) accession number: B8GF03

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program