B8GEE1 (B8GEE1_METPE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase HAMAP MF_01208
Short name=OPRT HAMAP MF_01208
Short name=OPRTase HAMAP MF_01208
EC=2.4.2.10 HAMAP MF_01208

Gene names

Name:	pyrE HAMAP MF_01208
Ordered Locus Names:	Mpal_2360

Organism

Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]

Taxonomic identifier

521011 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily. HAMAP MF_01208

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis HAMAP MF_01208
Ligand	Magnesium HAMAP MF_01208
Molecular function	Glycosyltransferase HAMAP MF_01208 EMBL ACL17642.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	113 – 121	9	5-phosphoribose 1-diphosphate binding By similarity HAMAP MF_01208
Sites
	Binding site	88	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	92	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	94	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	117	1	Orotate By similarity HAMAP MF_01208
	Binding site	145	1	Orotate By similarity HAMAP MF_01208

Sequences

	Sequence		Length	Mass (Da)
	B8GEE1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 3815BEC9ED79E51C	FASTA	172	17,952
	10 20 30 40 50 60 MVNEKTITEL LIDYKAIEFG DFILASGAIS RYYIDIKTAM THPALLNRVG TEFSERGSFD 70 80 90 100 110 120 VVAGVAVGGV PLAVATGLAA GKEYAIVRSA ARDHGKAGVV IGDVKGRKVL LVEDVTTSGG 130 140 150 160 170 SALYGVEQLR AAGATVTTVM TVVDREQGAV EALAAVGVRL VPLVRVSELL HL

References

[1]

"Complete sequence of Candidatus Methanosphaerula E1-9c." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001338 Genomic DNA. Translation: ACL17642.1.
RefSeq	YP_002467365.1. NC_011832.1.
3D structure databases
ProteinModelPortal	B8GEE1.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GEE1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7272081.
GenomeReviews	Gene locus Mpal_2360 in contig CP001338_GR.
KEGG	mpl:Mpal_2360.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404341.
OMA	QTCLVIN.
ProtClustDB	PRK13811.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8GEE1_METPE

Accession

Primary (citable) accession number: B8GEE1

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	November 16, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)