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Protein

NAD kinase

Gene

nadK

Organism
Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Proton acceptorUniRule annotation1
Binding sitei142NADUniRule annotation1
Binding sitei159NADUniRule annotation1
Binding sitei161NADUniRule annotation1
Binding sitei196NAD; via carbonyl oxygenUniRule annotation1
Binding sitei230NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi63 – 64NADUniRule annotation2
Nucleotide bindingi131 – 132NADUniRule annotation2
Nucleotide bindingi172 – 177NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Mpal_2339
OrganismiMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)
Taxonomic identifieri521011 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanosphaerula
Proteomesi
  • UP000002457 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001335761 – 270NAD kinaseAdd BLAST270

Interactioni

Protein-protein interaction databases

STRINGi521011.Mpal_2339.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01348. Archaea.
COG0061. LUCA.
HOGENOMiHOG000227222.
KOiK00858.
OMAiCAMNEAV.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B8GEC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLLMSRID SEETLAYTRT LGIDLENVGF QIIYDTATAS ALEVNGVPLS
60 70 80 90 100
SADPDAVVAI GGDGTILLCI QQMNIQRPII GINRGEVGFL ADLEPEEAFS
110 120 130 140 150
FLKELKPGFP VERRMRIDLS VDGKPLGTAL NEAVIVTSRP AKMLRFSVLV
160 170 180 190 200
DGVRAEQFRG DGLLISTPTG STAYAMSAGG PIIDPRIEGF LLVPLAPYML
210 220 230 240 250
SSRPHLICAT RTLAVRLEAS KPAHLVLDGQ RTIDLGDQAT IEMTRSPEPA
260 270
QFIDVNRNFF SKVEQKLRNL
Length:270
Mass (Da):29,392
Last modified:March 3, 2009 - v1
Checksum:i968C817E12C86B28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001338 Genomic DNA. Translation: ACL17623.1.
RefSeqiWP_012618942.1. NC_011832.1.

Genome annotation databases

EnsemblBacteriaiACL17623; ACL17623; Mpal_2339.
GeneIDi7272060.
KEGGimpl:Mpal_2339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001338 Genomic DNA. Translation: ACL17623.1.
RefSeqiWP_012618942.1. NC_011832.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi521011.Mpal_2339.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL17623; ACL17623; Mpal_2339.
GeneIDi7272060.
KEGGimpl:Mpal_2339.

Phylogenomic databases

eggNOGiarCOG01348. Archaea.
COG0061. LUCA.
HOGENOMiHOG000227222.
KOiK00858.
OMAiCAMNEAV.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADK_METPE
AccessioniPrimary (citable) accession number: B8GEC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.