Skip Header

Contribute Send feedback
Read comments (?) or add your own

B8GE24 (B8GE24_METPE) Unreviewed, UniProtKB/TrEMBL

Last modified November 16, 2011. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Adenylosuccinate synthetase HAMAP MF_00011
Short name=AMPSase HAMAP MF_00011
Short name=AdSS HAMAP MF_00011
EC=6.3.4.4 HAMAP MF_00011
Alternative name(s):
IMP--aspartate ligase HAMAP MF_00011
Gene names
Name:purA HAMAP MF_00011
Ordered Locus Names:Mpal_2232
OrganismMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) [Complete proteome] [HAMAP]
Taxonomic identifier521011 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanosphaerula

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity. RuleBase RU000520

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011

Catalytic activity

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011 RuleBase RU000520

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011 RuleBase RU000520

Subunit structure

Homodimer By similarity. HAMAP MF_00011

Subcellular location

Cytoplasm By similarity HAMAP MF_00011.

Sequence similarities

Belongs to the adenylosuccinate synthetase family. RuleBase RU004163 HAMAP MF_00011

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding26 – 327GTP By similarity HAMAP MF_00011
Nucleotide binding56 – 583GTP By similarity HAMAP MF_00011
Nucleotide binding295 – 2973GTP By similarity HAMAP MF_00011
Nucleotide binding335 – 3373GTP By similarity HAMAP MF_00011
Region27 – 304IMP binding By similarity HAMAP MF_00011
Region54 – 574IMP binding By similarity HAMAP MF_00011
Region263 – 2697Substrate binding By similarity HAMAP MF_00011

Sites

Active site271Proton acceptor By similarity HAMAP MF_00011
Active site571Proton donor By similarity HAMAP MF_00011
Metal binding271Magnesium By similarity HAMAP MF_00011
Metal binding561Magnesium; via carbonyl oxygen By similarity HAMAP MF_00011
Binding site1381IMP By similarity HAMAP MF_00011
Binding site1521IMP; shared with dimeric partner By similarity HAMAP MF_00011
Binding site1901IMP By similarity HAMAP MF_00011
Binding site2051IMP By similarity HAMAP MF_00011
Binding site2671IMP By similarity HAMAP MF_00011
Binding site2691GTP By similarity HAMAP MF_00011

Sequences

Sequence LengthMass (Da)Tools
B8GE24 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 5A98CE5610167BAC

FASTA35037,804
        10         20         30         40         50         60 
MFIRVNHPTV YDRIMTCTVI VGGFFGDEGK GKIVAHIAHA DEPTIIARGG VGPNAGHTVQ 

        70         80         90        100        110        120 
VGEKEYGVRM VPSGFVYPDA RLCIGAGVLV DPRVLAREVD VLGVKGRIFV DRRCGIIEEK 

       130        140        150        160        170        180 
HIAADKASVH LSQKIGSTGT GCGPANADRV MRVGRLAQDL PELKEYLIDV PVEVNTAIDN 

       190        200        210        220        230        240 
DEVVLLEGTQ GFGISLYYGS YPYVTSKDTS ASQIAADIGV GPTGIDDVVV VFKAYPTRVG 

       250        260        270        280        290        300 
EGPFSTEMSH EQSGELGIQE FGTVTHRERR IGCWDGKMAR YSAMINGCTQ VAITGIDKVD 

       310        320        330        340        350 
KACFGVTDYE KLTPKALDFL RAAEDDINRP ITLISTGPEL SQIIDLRDEF

« Hide

References

[1]"Complete sequence of Candidatus Methanosphaerula E1-9c."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Lu M., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.O., Zinder S.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1556 / DSM 19958 / E1-9c.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001338 Genomic DNA. Translation: ACL17525.1.
RefSeqYP_002467248.1. NC_011832.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB8GE24.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7272529.
GenomeReviewsGene locus Mpal_2232 in contig CP001338_GR.
KEGGmpl:Mpal_2232.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG658237.
OMAYVLGIIK.
ProtClustDBPRK04293.

Family and domain databases

HAMAPMF_00011. Adenylosucc_synth.
[Tree]
InterProIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
[Graphical view]
KOK01939.
PANTHERPTHR11846. Asucc_synthtase. 1 hit.
PfamPF00709. Adenylsucc_synt. 2 hits.
[Graphical view]
SMARTSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
PROSITEPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB8GE24_METPE
AccessionPrimary (citable) accession number: B8GE24
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: November 16, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info