B8GD86 (B8GD86_CHLAD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022

Alternative name(s):
Glutamyl-tRNA synthetase HAMAP MF_00022

Gene names

Name:	gltX HAMAP MF_00022
Ordered Locus Names:	Cagg_2273

Organism

Chloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP] EMBL ACL25153.1

Taxonomic identifier

326427 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00022
Cellular component	Cytoplasm HAMAP MF_00022
Ligand	ATP-binding HAMAP MF_00022 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00022 EMBL ACL25153.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	14 – 24	11	"HIGH" region By similarity HAMAP MF_00022
	Motif	261 – 265	5	"KMSKS" region By similarity HAMAP MF_00022
Sites
	Binding site	264	1	ATP By similarity HAMAP MF_00022

Sequences

	Sequence		Length	Mass (Da)
	B8GD86 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: C7B8A05236C072E5	FASTA	503	57,000
	10 20 30 40 50 60 MAGIEGPVRV RFAPSPTGSL HIGGVRTALF NWLCARHYGG QFILRIEDTD EKRFVPGAAD 70 80 90 100 110 120 DISISLRWVG IDWDEGPDVG GPYGPYVQSQ RFEQGIYRPY VDQLLEAGLA YMSFTTEEEL 130 140 150 160 170 180 TQMRAAAEAA GIKAFRFRGS ERDWPLERQR ELAASGKPYT VRLKTPLDGE TRFSDLIRGG 190 200 210 220 230 240 DEIVVQNDQL QDIVLIKSSG MPVYHFAHLV DDHLMKITLV MRGEEWVPST PYHVLLYDYF 250 260 270 280 290 300 GWQRPIFAHL PAILRHDGKG KLSKRKDDVA TQRFRERGYL PETMLNYLAL QGWSYDGVTE 310 320 330 340 350 360 IMNREELIAR FSIERIQPSP ARWNPEKLRD MNGIYIRKLS REQLAERVLP FMQRAGLIGD 370 380 390 400 410 420 PASDTERAYL LQLIPLIHER LEELQEAPEL LEFFYCEVTP GVTYQPTDLI PKKHDAVQTV 430 440 450 460 470 480 TMLRAAHDVL QRQTDWTAPA LETALRTLVE QLGVKPGPLF SAIRIAVTGR SVAPPLFDTL 490 500 AGVGRERSLE RLAAAIAALE QMS

References

[1]

"Complete sequence of Chloroflexus aggregans DSM 9485." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001337 Genomic DNA. Translation: ACL25153.1.
RefSeq	YP_002463589.1. NC_011831.1.
3D structure databases
ProteinModelPortal	B8GD86.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GD86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7266686.
GenomeReviews	Gene locus Cagg_2273 in contig CP001337_GR.
KEGG	cag:Cagg_2273.
PATRIC	21407151. VBIChlAgg85409_2353.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8GD86_CHLAD

Accession

Primary (citable) accession number: B8GD86

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)