B8GC03 (FTHS_CHLAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Cagg_0024

Organism

Chloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP]

Taxonomic identifier

326427 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_1000185249
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8GC03 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 73C749299683B33F	FASTA	572	60,269
	10 20 30 40 50 60 MKTSLQIAAE AHLEPIGVIA ERLGLPTEYL EPYGRYRGKI DLTFLDDHAN RPRGRYILVS 70 80 90 100 110 120 AITPTPLGEG KTTTAIGLAM ALNRIGKRAV VTLRQSSLGP VFGIKGGGAG GGYSQIVPLA 130 140 150 160 170 180 ESILHLNGDI HAVSQAHNQL AALTDNSWYH GNPLDIDPDR IEIRRVVDVN DRFLRQVMIG 190 200 210 220 230 240 LGGKQNGFPR QTGFDISVAS ELMAILAMVS GAGAKAALRE LRARIGRMVV AFRRDGTPVT 250 260 270 280 290 300 AEDVRGAGAA TVLMREALKP NLMQTIENTP ALIHAGPFAN IAQGNSSILA DLVALRCAEY 310 320 330 340 350 360 TITEAGFGAD IGAEKFFNLK CRAGGLWPDA AVIVATVRAL KAHSGKYEIV AGKPLPVALL 370 380 390 400 410 420 QENPDDVFAG GDNLRRQIAN ITQFGVPVVV ALNTYPEDTA TEIEAVAQIA TAAGAVGMAV 430 440 450 460 470 480 SNVYAAGGAG GVELAKLVAR VTERPGPREP KYLYPLEMPL AEKIEVIARR IYGAAGIELS 490 500 510 520 530 540 ATAAAQLATL TEAGFGNLPI CMVKTHLSLS HDPKLRGAPA GFIFPIREVR ISAGAGFILP 550 560 570 IAGTTVTMPG LGAHPAAHQV DIDDDGNIVG LF

References

[1]

"Complete sequence of Chloroflexus aggregans DSM 9485." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001337 Genomic DNA. Translation: ACL22977.1.
RefSeq	YP_002461413.1. NC_011831.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8GC03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7269021.
GenomeReviews	Gene locus Cagg_0024 in contig CP001337_GR.
KEGG	cag:Cagg_0024.
PATRIC	21402430. VBIChlAgg85409_0025.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG677721.
OMA	EIMAVLC.
ProtClustDB	CLSK983690.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CHLAD

Accession

Primary (citable) accession number: B8GC03

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program